Efficiency of Artemisia annua L. essential oil and its chitosan/tripolyphosphate or zeolite encapsulated form in controlling Sitophilus oryzae L.

The rice weevil, Sitophilus oryzae L., is one of the most widespread and destructive stored-product pests and resistant to a wide range of chemical insecticides. In this research, Artemisia annua L. essential oil (EO) and its encapsulated form by chitosan/TPP (tripolyphosphate) and zeolite were tested against S. oryzae adults. The order of toxicity was chitosan/TPP (LC30: 30.83, LC50: 39.52, and LC90: 72.50 μL/L air) > pure EO (LC30: 35.75, LC50: 46.25, and LC90: 86.76 μL/L air) > EO loaded in the zeolite (LC30: 43.35, LC50: 55.07, and LC90: 98.80 μL/L air). These encapsulated samples were characterized by dynamic light scattering (DLS) and field emission scanning electron microscope (FE-SEM) which revealed the size and morphology of the droplets measuring 255.2 to 272 nm and 245 to 271.8 nm for EO loaded in chitosan and zeolite respectively. The encapsulation efficiency and loading percentages of A. annua EO in chitosan/TPP and zeolite were 40.16% and 6.01%, and 88% and 85%, respectively. Fumigant persistence was increased from 6 days for pure EO then, 20 and 22 days for encapsulated oil in zeolite and chitosan/TPP, respectively. Our results showed that A. annua EO contains (±)-camphor (29.29%), 1,8-cineole (12.56%), β-caryophyllene (10.29%), α-pinene (8.68%), and artemisia ketone (8.48%) as its major composition. The activity level of glutathione S-transferase increased while general esterase and acetylcholinesterase activity were significantly inhibited in the treated group compared with the control. Antioxidant enzymes, including catalase, peroxidase, and superoxide dismutase were activated in treated adults compared to controls. The current results suggest that encapsulation of A. annua EO by chitosan/TPP and zeolite in addition to safety and environmentally friendly approach could increase its sustainability and therefore enhancing the efficiency in controlling S. oryzae in storage.