LINC00707 inhibits myocardial fibrosis and immune disorder in rheumatic heart disease by regulating miR-145-5p/S1PR1

免疫印迹 免疫系统 心肌纤维化 心脏病 免疫学 纤维化 发病机制 分子生物学 医学 生物 内科学 基因 生物化学
作者
Wen Zhao,Huang Guo-xiong,Jiemei Ye
出处
期刊:Biotechnology & Genetic Engineering Reviews [Taylor & Francis]
卷期号:40 (4): 3073-3086 被引量:5
标识
DOI:10.1080/02648725.2023.2204598
摘要

LINC00707 is a lncRNA that can regulate a variety of diseases. This study mainly investigated that the expression of LINC00707 in rheumatic heart disease (RHD) and LINC00707 regulates S1PR1 by targeting miR-145-5p to inhibit myocardial fibrosis and immune disorder in RHD. A rat model of RHD induced by inactivated group A β-hemolytic streptococcus (GSA) was established. Sixty female Lewis rats (8 weeks of age) were randomly divided into six groups, including control (Con), RHD, RHD+NC, RHD+LINC00707, RHD+miR-145-5p and RHD+LINC00707+miR-145-5p. The mRNA expression was detected by Quantitative Real-time polymerase chain reaction (qRT-PCR). Protein expression of S1PR1 was detected by western blot. The levels of myocardial damage markers (CK-MB, cTnl) and inflammatory immune markers (IL-6, IL-17 and IL-21) were measured by enzyme linked immunosorbent assay (ELISA). The Collagen III/I(COLIII/I) ratio, mRNA expression of COLIIIα1 and FSP1 of rat heart valve tissue in the RHD group was observably higher by comparison with the CON group. The expression of LINC00707 was observably lower in the RHD group. LINC00707 inhibited myocardial fibrosis and immune disorder in RHD. MiR-145-5p was the target gene of LINC00707 via Targetscan prediction. Luciferase reporter experiment confirmed that miR-145-5p was directly regulated by LINC00707. The expression of miR-145-5p in the RHD group was observably higher by comparison with the CON group and LINC00707 observably decreased the expression of miR-145-5p. miR-145-5p mimic reversed the inhibiting effect of LINC00707 on myocardial fibrosis and immune disorder. Furthermore, S1PR1 was confirmed to be downstream gene of miR-145-5p and low expressed in the RHD model. LINC00707 could inhibit myocardial fibrosis and immune disorder in RHD by regulating miR-145-5p/S1PR1.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Zxx关注了科研通微信公众号
刚刚
刚刚
franca2005完成签到 ,获得积分10
刚刚
本末倒纸完成签到 ,获得积分10
2秒前
wbscz应助星辰采纳,获得10
2秒前
toxikon发布了新的文献求助10
3秒前
4秒前
6秒前
大模型应助剁辣椒蒸鱼头采纳,获得20
6秒前
小北完成签到 ,获得积分10
6秒前
7秒前
高挑的冰露完成签到 ,获得积分10
10秒前
ruochenzu发布了新的文献求助10
10秒前
老李完成签到,获得积分10
10秒前
11秒前
12秒前
tough_cookie完成签到 ,获得积分10
13秒前
彩钢房完成签到,获得积分10
14秒前
MeSs完成签到 ,获得积分10
15秒前
toxikon完成签到,获得积分10
16秒前
一点通完成签到,获得积分10
16秒前
Lei完成签到,获得积分10
17秒前
17秒前
17秒前
常若冰完成签到,获得积分10
17秒前
纯真的元风完成签到,获得积分10
18秒前
哇哈哈哈完成签到,获得积分10
18秒前
清秋1001完成签到 ,获得积分10
19秒前
qq完成签到,获得积分10
20秒前
荒野风发布了新的文献求助10
21秒前
Zxx发布了新的文献求助10
22秒前
23秒前
23秒前
确幸完成签到 ,获得积分10
23秒前
苒苒完成签到,获得积分10
23秒前
24秒前
酷波er应助c123采纳,获得10
24秒前
TIAOTIAO完成签到,获得积分10
26秒前
未晚完成签到 ,获得积分10
26秒前
27秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038619
求助须知:如何正确求助?哪些是违规求助? 3576294
关于积分的说明 11375058
捐赠科研通 3306084
什么是DOI,文献DOI怎么找? 1819374
邀请新用户注册赠送积分活动 892698
科研通“疑难数据库(出版商)”最低求助积分说明 815066