circRNAs deregulation in exosomes derived from BEAS-2B cells is associated with vascular stiffness induced by PM2.5

竞争性内源性RNA 粘合连接 小RNA 细胞生物学 生物 表型 发病机制 化学 下调和上调 免疫学 基因 钙粘蛋白 细胞 长非编码RNA 生物化学
作者
Qingping Liu,Yaling Zhang,Bin Han,Mengruo Wang,Huaifang Hu,Jie Ning,Wentao Hu,Mei‐Yu Chen,Yaxian Pang,Yuanyuan Chen,Lei Bao,Yujie Niu,Rong Zhang
出处
期刊:Journal of Environmental Sciences-china [Elsevier]
卷期号:137: 527-539 被引量:1
标识
DOI:10.1016/j.jes.2023.02.027
摘要

As an environmental pollutant, ambient fine particulate matter (PM2.5) was linked to cardiovascular diseases. The molecular mechanisms underlying PM2.5-induced extrapulmonary disease has not been elucidated clearly. In this study the ambient PM2.5 exposure mice model we established was to explore adverse effects of vessel and potential mechanisms. Long-term PM2.5 exposure caused reduced lung function and vascular stiffness in mice. And chronic PM2.5 induced migration and epithelial-mesenchymal transition (EMT) phenotype in BEAS-2B cells. After PM2.5 treatment, the circRNAs and mRNAs levels of exosomes released by BEAS-2B cells were detected by competing endogenous RNA (ceRNA) array, which contained 1664 differentially expressed circRNAs (DE-circRNAs) and 308 differentially expressed mRNAs (DE-mRNAs). By bioinformatics analysis on host genes of DE-circRNAs, vascular diseases and some pathways related to vascular diseases including focal adhesion, tight junction and adherens junction were enriched. Then, ceRNA network was constructed, and DE-mRNAs in ceRNA network were conducted functional enrichment analysis by Ingenuity Pathway Analysis, which indicated that hsa_circ_0012627, hsa_circ_0053261 and hsa_circ_0052810 were related to vascular endothelial dysfunction. Furthermore, it was verified experimentally that ExoPM2.5 could induce endothelial dysfunction by increased endothelial permeability and decreased relaxation in vitro. In present study, we investigated in-depth knowledge into the molecule events related to PM2.5 toxicity and pathogenesis of vascular diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
an完成签到,获得积分10
1秒前
1秒前
庞威完成签到 ,获得积分10
3秒前
李健的粉丝团团长应助ZzZz采纳,获得10
3秒前
科目三应助优秀醉易采纳,获得10
5秒前
Lucas发布了新的文献求助10
5秒前
专注的水壶完成签到 ,获得积分10
6秒前
不可思宇完成签到,获得积分10
8秒前
ljy阿完成签到 ,获得积分10
8秒前
9秒前
鳗鱼鸽子完成签到,获得积分10
9秒前
屁颠屁颠_狼完成签到 ,获得积分0
12秒前
烂漫的无剑完成签到,获得积分10
12秒前
草莓熊和他的豆角完成签到,获得积分10
12秒前
文献狂人发布了新的文献求助10
12秒前
高级后勤完成签到,获得积分10
13秒前
松绿格完成签到 ,获得积分10
14秒前
babao完成签到,获得积分20
14秒前
虚幻谷波完成签到,获得积分10
14秒前
e394282438完成签到,获得积分10
15秒前
ytong完成签到,获得积分10
15秒前
Forever完成签到,获得积分10
16秒前
R喻andom发布了新的文献求助10
16秒前
多发paper啊完成签到,获得积分10
16秒前
管理想完成签到,获得积分10
16秒前
乐观的凌兰完成签到 ,获得积分10
17秒前
17秒前
18秒前
dream完成签到,获得积分10
18秒前
19秒前
lhh0529yt完成签到,获得积分10
20秒前
zlx完成签到,获得积分10
21秒前
山茶花白玫瑰完成签到 ,获得积分10
22秒前
Brian完成签到,获得积分10
22秒前
ZzZz发布了新的文献求助10
22秒前
witty完成签到,获得积分10
22秒前
mm_zxh完成签到,获得积分10
22秒前
词汇过万完成签到,获得积分10
23秒前
干秋寒完成签到,获得积分10
23秒前
Qing发布了新的文献求助10
23秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2024 Medicinal Chemistry Reviews 400
Dictionary of socialism 350
Mixed-anion Compounds 300
Geochemistry, 2nd Edition 地球化学经典教科书第二版 300
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3196879
求助须知:如何正确求助?哪些是违规求助? 2845528
关于积分的说明 8054887
捐赠科研通 2510263
什么是DOI,文献DOI怎么找? 1342345
科研通“疑难数据库(出版商)”最低求助积分说明 639355
邀请新用户注册赠送积分活动 608690