内胚层
极地的
内质网
生物
生长素极性运输
化学
生物物理学
生物化学
运输机
亚细胞定位
海熊
细胞生物学
氨基酸
拟南芥
基因
物理
天文
突变体
作者
Noriko Konishi,Namiki Mitani‐Ueno,Naoki Yamaji,Jian Feng
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2023-03-09
卷期号:35 (6): 2232-2250
被引量:7
标识
DOI:10.1093/plcell/koad073
摘要
Abstract Silicon (Si) is important for stable and high yields in rice (Oryza sativa), a typical Si hyperaccumulator. The high Si accumulation is achieved by the cooperation of 2 Si transporters, LOW SILICON 1 (OsLsi1) and OsLsi2, which are polarly localized in cells of the root exodermis and endodermis. However, the mechanism underlying their polar localization is unknown. Here, we identified amino acid residues critical for the polar localization of OsLsi1. Deletion of both N- and C-terminal regions resulted in the loss of its polar localization. Furthermore, the deletion of the C-terminus inhibited its trafficking from the endoplasmic reticulum to the plasma membrane. Detailed site-directed mutagenesis analysis showed that Ile18 at the N-terminal region and Ile285 at the C-terminal region were essential for the polar localization of OsLsi1. Moreover, a cluster of positively charged residues at the C-terminal region is also required for polar localization. Phosphorylation and Lys modifications of OsLsi1 are unlikely to be involved in its polar localization. Finally, we showed that the polar localization of OsLsi1 is required for the efficient uptake of Si. Our study not only identified critical residues required for the polar localization of OsLsi1, but also provided experimental evidence for the importance of transporter polarity for efficient nutrient uptake.
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