Self-powered biosensing platform for Highly sensitive detection of soluble CD44 protein

化学 适体 生物传感器 检出限 胆红素氧化酶 线性范围 纳米技术 电极 CD44细胞 葡萄糖氧化酶 靶蛋白 色谱法 生物化学 分子生物学 细胞 材料科学 物理化学 基因 生物
作者
Shanshan Sun,Meng Su,Han Xiao,Xiaoshuang Yin,Ying Liu,Wenzhong Yang,Yun Chen
出处
期刊:Talanta [Elsevier BV]
卷期号:272: 125824-125824
标识
DOI:10.1016/j.talanta.2024.125824
摘要

In this study, a self-powered biosensor based on an enzymatic biofuel cell was proposed for the first time for the ultrasensitive detection of soluble CD44 protein. The as-prepared biosensor was composed of the co-exist aptamer and glucose oxidase bioanode and bilirubin oxidase modified biocathode. Initially, the electron transfer from bioanode to biocathode was hindered due to the presence of the aptamer with high insulation, generating a low open-circuit voltage (EOCV). Once the target CD44 protein was present, it was recognized and captured by the aptamer at the bioanode, thus the interaction between the target CD44 protein and the immobilized aptamer caused the structural change at the surface of the electrode, which facilitated the transfer of electrons. The EOCV showed a good linear relationship with the logarithm of the CD44 protein concentrations in the range of 0.5–1000 ng mL−1 and the detection limit was 0.052 ng mL−1 (S/N = 3). The sensing platform showed excellent anti-interference performance and outstanding stability that maintained over 97% of original EOCV after 15 days. In addition, the relative standard deviation (1.40–1.96%) and recovery (100.23–101.31%) obtained from detecting CD44 protein in real-life blood samples without special pre-treatment indicated that the constructed biosensor had great potential for early cancer diagnosis.
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