里氏木霉
纤维素酶
菌丝体
CDC42型
GTP酶
生物
分生孢子
木霉菌
基因
异位表达
细胞生物学
表型
细胞外
菌丝
微生物学
酶
生物化学
生物技术
毒力
植物
作者
Fangting Jiang,Jiudong Tian,Jie Yuan,Shengjie Wang,Tongtong Bao,Qiuhui Chen,Le Gao,Jinyang Li,Lijuan Ma
出处
期刊:Fermentation
[MDPI AG]
日期:2023-12-28
卷期号:10 (1): 26-26
被引量:1
标识
DOI:10.3390/fermentation10010026
摘要
The small GTPases of the Rho family are known to regulate various biological processes in filamentous fungi. In this study, we investigated the impact of deleting Rho proteins on the growth and cellulase production of Trichoderma reesei. Our findings revealed that deletion of cdc42 led to the most severe growth defect and impaired cellulase production. Conversely, overexpression of cdc42 resulted in a hyperbranched phenotype, significantly enhancing cellulase production. Furthermore, the cdc42-overexpressing (OCdc42) strain showed an increased expression of multiple cellulase genes and Rho GTPase genes. Analysis of the secretome in the OCdc42 strain unveiled an increased abundance and diversity of extracellular proteins compared to the parent strain. These discoveries provide valuable insights into the functionality of Rho GTPases in T. reesei and offer potential targets for engineering fungi to improve plant biomass deconstruction in biorefineries.
科研通智能强力驱动
Strongly Powered by AbleSci AI