化学
生物素化
核糖核酸
活体细胞成像
光敏剂
单线态氧
亚细胞定位
生物物理学
生物化学
细胞生物学
细胞质
细胞
氧气
光化学
基因
生物
有机化学
作者
Jiying Liang,Jinghua Han,Xutao Gao,Ju‐Guang Han,Ran Li,Edmund C. M. Tse,Ying Li
标识
DOI：10.1021/acs.analchem.3c03614
摘要
Although APEX2-mediated proximity labeling has been extensively implemented for studying RNA subcellular localization in live cells, the biotin-phenoxyl radical used for labeling RNAs has a relatively low efficiency, which can limit its compatibility with other profiling methods. Herein, a set of phenol derivatives were designed as APEX2 probes through balancing reactivity, hydrophilicity, and lipophilicity. Among these derivatives, Ph_N
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