小桶
小RNA
生物
转录组
信号转导
基因
PI3K/AKT/mTOR通路
转化生长因子β
细胞生物学
转化生长因子
小RNA
核糖核酸
生物途径
炎症
计算生物学
基因表达
癌症研究
遗传学
免疫学
作者
Yuhang Li,Qianqian Ren,Jinpeng Wang,Xingping Wang,Zhuoma Luoreng,Yun Ma,Dawei Wei
标识
DOI:10.1016/j.rvsc.2022.10.011
摘要
MicroRNAs (miRNAs) are involved in the regulation of a variety of biological processes. However, the research on the regulatory role of bovine mammary epithelial cells (bMECs) is scarce. To date, there are no reports about the role of miR-199a-3p in bMECs. In this study, RNA sequencing (RNA-seq) technology was used to detect the transcriptomes of the miR-199a-3p overexpression and negative control (NC) groups of bMECs. Then, the screening and functional annotation of differentially expressed genes (DEGs) were conducted. The results showed that there were 140 DEGs (109 up-regulated and 31 down-regulated) in the miR-199a-3p overexpression group. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that the DEGs might regulate the immune and inflammatory responses via the phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway, transforming growth factor-beta (TGF-β) signaling pathway, and interleukin-17 (IL-17) signaling pathway, which revealed that miR-199a-3p might participate in regulating bMECs inflammation via affecting the expression of related genes and the above signaling pathways. This study may provide a new reference for potential therapeutic targets of cow mastitis.
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