化学
高尔基体
谷胱甘肽
细胞器
荧光
生物物理学
细胞内
赫拉
细胞毒性
分泌物
荧光寿命成像显微镜
生物化学
细胞
体外
酶
生物
物理
量子力学
作者
Aikun Liu,Haojie Cai,Zhibing Xu,Jinlei Li,Xiaoyu Weng,Changrui Liao,Jun He,Liwei Liu,Yiping Wang,Junle Qu,Hao Li,Jun Song,Jun Song
出处
期刊:Talanta
[Elsevier]
日期:2023-07-01
卷期号:259: 124520-124520
被引量:12
标识
DOI:10.1016/j.talanta.2023.124520
摘要
Glutathione (GSH) is present in almost every cell in the body and plays various integral roles in many biological processes. The Golgi apparatus is a eukaryotic organelle for the biosynthesis, intracellular distribution, and secretion of various macromolecules; however, the mechanism of GSH in the Golgi apparatus has not been fully elucidated. Here, specific and sensitive sulfur-nitrogen co-doped carbon dots (SNCDs) with orange-red fluorescence was synthesized for the detection of GSH in the Golgi apparatus. The SNCDs have a Stokes shift of 147 nm and excellent fluorescence stability, and they exhibited excellent selectivity and high sensitivity to GSH. The linear response of the SNCDs to GSH was in the range of 10–460 μM (LOD = 0.25 μΜ). More importantly, we used SNCDs with excellent optical properties and low cytotoxicity as probes, and successfully realized golgi imaging in HeLa cells and GSH detection at the same time.
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