纳米片
荧光团
生物传感器
化学
脱氧核糖核酸酶ⅰ
脱氧核糖核酸酶
核酸酶
荧光
猝灭(荧光)
检出限
DNA
纳米技术
组合化学
生物物理学
生物化学
色谱法
材料科学
有机化学
生物
物理
量子力学
基序列
作者
Guibin Peng,Bixia Lin,Manli Guo,Yujuan Cao,Ying Yu,Yumin Wang
出处
期刊:Talanta
[Elsevier]
日期:2023-07-01
卷期号:259: 124533-124533
标识
DOI:10.1016/j.talanta.2023.124533
摘要
Deoxyribonuclease I (DNase I) is a typical nuclease that plays key roles in many physiological processes and the development of a novel biosensing strategy for DNase I detection is of fundamental significance. In this study, a fluorescence biosensing nanoplatform based on a two-dimensional (2D) titanium carbide (Ti3C2) nanosheet for sensitive and specific detection of DNase I was reported. Fluorophore-labeled single-stranded DNA (ssDNA) can be spontaneously and selectively adsorbed on Ti3C2 nanosheet through the hydrogen bond and metal chelate interaction between phosphate groups of ssDNA and titanium of Ti3C2 nanosheet, resulting in effective quenching of the fluorescence emitted by fluorophore. Notably, it was found the enzyme activity of DNase I will be terminated by the Ti3C2 nanosheet. Therefore, the fluorophore-labeled ssDNA was firstly digested by DNase I and the “post-mixing” strategy of Ti3C2 nanosheet was chosen to evaluate the enzyme activity of DNase I, which provided the possibility of improving the accuracy of the biosensing method. Experimental results demonstrated that this method can be utilized for quantitative analysis of DNase I activity and exhibited a low detection limit of 0.16 U/ml. Additionally, the evaluation of DNase I activity in human serum samples and the screening of inhibitors with this developed biosensing strategy were successfully realized, implying that it has high potential as a promising nanoplatform for nuclease analysis in bioanalytical and biomedical fields.
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