化学
糖肽
糖蛋白组学
亲水作用色谱法
辣根过氧化物酶
介孔二氧化硅
色谱法
介孔材料
高效液相色谱法
有机化学
生物化学
酶
催化作用
抗生素
作者
Nianrong Sun,Jiawen Wang,Jizong Yao,Chunhui Deng
标识
DOI:10.1021/acs.analchem.6b04054
摘要
Hydrophilic interaction liquid chromatography (HILIC) is a significant enrichment strategy in glycoproteomics profiling. In this report, hydrophilic magnetic mesoporous silica materials (denoted as Fe3O4@mSiO2-IDA) were designed and synthesized as an outstanding enrichment platform for glycopeptide analysis. By taking advantage of their merits, such as large surface area, excellent hydrophilicity and unbiased affinity toward all types of glycopeptides, the Fe3O4@mSiO2-IDA nanomaterials were successfully applied to capture glycopeptides from complex samples. A total of 25 glycopeptides from horseradish peroxidase (HRP) digests and 33 glycopeptides from IgG were identified, respectively. Especially, as a result, 424 glycopeptides assigned to 140 glycoproteins were identified from only 2 μL human serum.
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