OBJECTIVE To study water-soluble compounds from fungus garden of Odontotermes formosanus. METHODS The chemical constituents of fungus garden were analyzed and identified by GC-MS. RESULTS 28 compounds were separated and 11 chemical constituents were identified. CONCLUSION The main constituents in water-solubles from fungus garden of Odontotermes formosanus are palmitic acid, linolei acid and oleic aid.