单克隆抗体
表位
化学
表位定位
胰岛素样生长因子结合蛋白
分子生物学
胰岛素样生长因子
生长因子
免疫沉淀
抗体
结合位点
结合蛋白
生物
生物化学
受体
免疫学
基因
作者
Schuller Ag,Lindenbergh-Kortleve Dj,de Boer Wi,Zwarthoff Ec,Drop Sl
出处
期刊:PubMed
日期:1993-03-01
卷期号:3 (1): 32-4
被引量:4
摘要
In order to identify regions in insulin-like growth factor binding protein-1 involved in the binding of IGFs, we tested three monoclonal antibodies, designated MAb A, B, and C on their interference with IGF-binding. Monoclonal A interfered with the binding of IGF to IGFBP-1 as determined by immunoprecipitation whereas monoclonal B and C did not. Furthermore MAb A was found to abolish IGFBP-1 inhibition of IGF stimulation in an in vitro proliferation assay. The epitopes of all three monoclonal antibodies were found to be located within the C-terminal part of IGFBP-1. The regions surrounding residue 188-196 and 222-227 are especially important for antibody recognition. These results indicate that MAb A functionally interferes with the binding of IGF to IGFBP-1. Furthermore, we suggest that part of the epitope of MAb A is located at or sterically near the IGF binding domain of IGFBP-1.
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