分散性
纳米颗粒
寡核苷酸
化学
聚合物
Zeta电位
纳米技术
核酸
PEG比率
费斯特共振能量转移
荧光
高分子化学
材料科学
DNA
有机化学
生物化学
经济
财务
物理
量子力学
作者
Antons Sizovs,Xianzhou Song,M. Neal Waxham,Yilong Jia,Fude Feng,Jianwei Chen,Amanda C. Wicker,Jianming Xu,Yan Yu,Jin Wang
摘要
Advancement of RNAi therapies is mainly hindered by the development of efficient delivery vehicles. The ability to create small size (<30 nm) oligonucleotide nanoparticles is essential for many aspects of the delivery process but is often overlooked. In this report, we describe diblock star polymers that can reproducibly complex double-stranded oligonucleotides into monodisperse nanoparticles with 15, 23, or 30 nm in diameter. The polymer–nucleic acid nanoparticles have a core–shell architecture with dense PEG brush coating. We characterized these nanoparticles using ITC, DLS, FRET, FCS, TIRF, and TEM. In addition to small size, these nanoparticles have neutral zeta-potentials, making the presented polymer architecture a very attractive platform for investigation of yet poorly studied polyplex size range for siRNA and antisense oligonucleotide delivery applications.
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