Chemical constituents from whole herb of Hedyotis scandens

Objective To investigate the chemical constituents in the whole herb of Hedyotis scandens.Methods The chemical constituents were isolated and identified by column chromatography on silica gel,ODS,Sephadex LH-20,as well as RP-HPLC.Their structures were elucidated on the basis of physicochemical properties and spectral analyses.Results Thirteen compounds were isolated from H.scandens and structures were identified as oleanolic acid(1),2α,3α,24-trihydroxy-olea-12-en-28-oic acid(2),2α,3β,24-trihydroxy-olea-12-en-28-oic acid(3),2α,3β-dihydroxy-urs-12-en-28-oic acid(4),2α,3β,19α-trihydroxy-urs-12-en-28-oic acid(5),betulinic acid(6),kaempferol-3-O-β-D-glucoside(7),quercetin(8),apigenin(9),apigenin-7-O-β-D-glucoside(10),luteolin(11),luteolin-7-O-β-D-glucoside(12),and daucosterol(13),respectively.Conclusion Compounds 1—13 are isolated from H.scandens for the first time.