低温保护剂
脐带血
造血
低温保存
右旋糖酐
男科
生物
免疫学
化学
分子生物学
生物化学
医学
细胞生物学
干细胞
胚胎
作者
R Chen,M Zhu,L Huang,Yunpeng Liu,Y Xi
出处
期刊:Chinese Journal of Hematology
日期:2000-08-01
卷期号:21 (8): 412-3
摘要
To search for effective cryoprotectants that can be used in cord blood bank to cryopreserve hematopoietic cells.Four kinds of combined reagents were used to cryopreserve cord blood cells. The effects of these cryoprotectants on biological properties of hematopoietic cells were monitored and compared after the cells were refrigerated for 1 to 4 months. The detected biological properties included the number of total nucleated cells (TNC), CD(34)(+) cells and CFCs.The cryoprotectants of Dextran-40 + 10%DMSO resulted in the highest recovery rates, TNC, CD(34)(+) cells and CFCs were 77.8%, 78.5% and 87.2%, respectively. The combination of 10%DMSO + 6% AB serum + 5%HES led to the lowest recovery rates.Dextran-40 + 10%DMSO is an ideal cryoprotectant combination for cryopreserving hematopoietic cells in the cord blood bank.
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