化学
多路复用
检出限
荧光
磁选
DNA
磁性纳米粒子
互补DNA
纳米颗粒
纳米技术
色谱法
组合化学
材料科学
基因
生物化学
物理
生物
量子力学
冶金
生物信息学
作者
Jun Hu,Cong‐Ying Wen,Zhiling Zhang,Min Xie,Jiao Hu,Min Wu,Dai‐Wen Pang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2013-11-06
卷期号:85 (24): 11929-11935
被引量:67
摘要
In this study, we report a simple method for simultaneous detection of multiplex DNA sequences, including complementary DNA (cDNA) sequences of HIV and HCV, DNA sequence of HBV, with QDs-encoded fluorescent nanospheres and nano-γ-Fe2O3-coated magnetic nanospheres. Detection was achieved on a fluorescence spectrophotometer without additional auxiliary instruments, and the detection limit was about 100 pM. Here, QDs-encoded fluorescent nanospheres (FNS) with different photoluminescent properties, and magnetic nanospheres (MNS) were separately fabricated by stepwise assembly of hydrophobic QDs or nano-γ-Fe2O3 on the surface of branched poly(ethylene imine) (PEI)-coated nanospheres in precisely controlled amounts, finally followed by silica encapsulation. FNS-labeled probe DNAs and MNS-labeled capture DNAs were used to hybridize with the corresponding targets at the same time. After magnetic separation, the sandwich-structured adducts were measured by fluorescence spectrophotometry. The results indicated that the targets could be detected with high sensitivity. This method is convenient, fast enough, and capable of high anti-interference. Therefore, it is expected to be used for simultaneous detection and separation of multiple targets at high levels of purity and throughput.
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