免疫印迹
势垒函数
溃疡性结肠炎
结肠炎
细胞凋亡
医学
地穴
肠粘膜
化学
炎症性肠病
促炎细胞因子
肿瘤坏死因子α
炎症
内科学
免疫学
药理学
病理
生物
生物化学
细胞生物学
疾病
基因
作者
Shaojie Yin,Haifeng Yang,Ya Tao,Simin Wei,Liuhui Li,Mingjiang Liu,Jingui Li
出处
期刊:Inflammation
[Springer Nature]
日期:2020-01-02
卷期号:43 (2): 765-776
被引量:58
标识
DOI:10.1007/s10753-019-01164-1
摘要
There are very few reports on the protective effect of artesunate (ARS) in ulcerative colitis (UC). This study focused on the efficacy of ARS on intestinal barrier, inflammatory response, and potential mechanism in dextran sulfate sodium (DSS)-induced ulcerative colitis in mice. The results suggested that ARS treatment markedly alleviated DSS-induced clinical symptoms by relieving body weight loss, the disease activity index (DAI) score, and preventing colonic shortening. HE staining and scanning electron microscope analysis revealed that ARS treatment significantly protected the integrity of intestinal barrier through alleviating DSS-induced erosion of surface epithelial cells, reduction of goblet cells, and destruction of the crypt accompanied with inflammatory cells infiltration. Immunofluorescence histochemical staining and western blot assay confirmed that ARS notably inhibited the loss of Muc2 and claudin-1 in mucosal layer with a relative higher level of Bcl-2/Bax ratio and, moreover, inhibited cleaved-caspase-3 expression in colon tissue. In addition, this study reconfirmed the anti-inflammatory function of ARS evidenced by remarkably suppressing the phosphorylation of nuclear factor-κBα (IκBα) and NF-κB p65 and the expression of IL-1β, IL-6, and TNF-α while enhancing IL-10 expression. Taken together, these data highlight that ARS has the protective effect on UC through maintaining the expression of intestinal mucosal barrier-related proteins, suppressing the apoptosis and inflammatory response. This study may facilitate to understand the action mechanism of ARS against UC.
科研通智能强力驱动
Strongly Powered by AbleSci AI