纳米团簇
过氧化氢
检出限
化学
过氧化物酶
荧光
线性范围
核化学
葡萄糖氧化酶
DNA
生物传感器
无机化学
酶
色谱法
生物化学
有机化学
量子力学
物理
作者
Zhiwei Du,Chunying Wei
标识
DOI:10.1002/slct.202000956
摘要
Abstract Four kinds of DNA‐based Ag and Cu/Ag nanoclusters (NCs) were synthesized and their fluorescence properties were investigated at pH 7.0 and 5.5. Among them the Cu/Ag NCs templated by DNA containing both C‐rich and G‐rich sequences (the c‐myc TBA−Cu/Ag NCs) give the strongest and the most stable fluorescence emission at ∼555 nm under neutral and acidic conditions. Unexpectedly, the c‐myc TBA−Cu/Ag NCs can catalyze efficiently and rapidly the reaction of hydrogen peroxide (H 2 O 2 ) with 3,3′,5,5′‐tetramethylbenzidine (TMB) to produce blue TMB oxide (TMBox) under acidic conditions, indicating the high peroxidase‐mimicking activity. Based on this nanozyme the detection for H 2 O 2 and glucose was performed using colorimetric method. The results indicate that the linear range of detection for H 2 O 2 is from 0.1 to 1 mM with the detection limit (LOD) of 7.42 μM, while the linear range is from 0.1 to 0.7 mM and LOD was calculated to be 9.38 μM for glucose. Additionally, glucose can be successfully detected in diluted serum samples and has an acceptable recovery rate, indicating that this nanozyme has a potential application prospect.
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