Effector Repertoire of Phytophthora betacei: In Search of Possible Virulence Factors Responsible for Its Host Specificity

疫病疫霉菌 效应器 卵菌 生物 疫霉菌 毒力 大豆疫霉 基因 遗传学 植物 细胞生物学
作者
Paola Rojas-Estevez,David Urbina-Gómez,David A. Ayala-Usma,Natalia Guayazán‐Palacios,María Fernanda Mideros,Adriana Bernal,Martha Cárdenas,Silvia Restrepo
出处
期刊:Frontiers in Genetics [Frontiers Media]
卷期号:11 被引量:4
标识
DOI:10.3389/fgene.2020.00579
摘要

Phytophthora betacei is an oomycete plant pathogen closely related to Phytophthora infestans. It infects tree tomato (Solanum betaceum) in northern South America, but is, under natural conditions, unable to infect potatoes or tomatoes, the main hosts of its sister species P. infestans. We characterized, and compared the effector repertoires of P. betacei and other Phytophthora species. To this end, we used in silico approaches to predict and describe the repertoire of secreted proteins in Phytophthora species and determine unique and core effectors. P. betacei has the largest proteome and secretome of all Phytophthora species evaluated. We identified between 450 and 1933 candidate effector genes in P. ramorum, P. sojae, P. cactorum, P. parasitica, P. palmivora, P. infestans, and P. betacei genomes. The P. betacei predicted secretome contains 5653 proteins, 1126 of which are apoplastic effectors and 807cytoplasmic effectors. Genes encoding cytoplasmic effectors include 791 genes with an RxLR domain (the largest number known so far in a Phytophthora species) and 16 with a Crinkler (CRN) domain. We detected homologs of previously described avirulence gene (Avr) present in Phytophthora spp, such as Avr1, Avr3b, Avr4, and Avrblb1, suggesting a high level of effector gene conservation among Phytophthora species. Nonetheless, fewer CRN effectors were obtained in P. betacei compared to all other Phytophthora species analyzed. The comparison between P. infestans and P. betacei effector profiles shows unique features in P. betacei that might be involved in pathogenesis and host preference. Indeed, 402 unique predicted effector genes were detected in P. betacei, corresponding to 197 apoplastic effector genes, 203 RxLR cytoplasmic effector genes, and 2 effector genes with CRN domain. This is the first characterization of the effector profile of P. betacei and the broadest comparison of predicted effector repertoires in the genus Phytophthora following a standardized prediction pipeline. The resultant P. betacei putative effector repertoire provides a reasonable set of proteins whose experimental validation could lead to understand the specific virulence factors responsible for the host specificity of this species.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
所所应助yuyu采纳,获得10
2秒前
云海老完成签到,获得积分10
3秒前
jimskylxk完成签到,获得积分10
3秒前
Joker完成签到,获得积分20
3秒前
研友_VZG7GZ应助聪慧小霜采纳,获得10
4秒前
ding应助聪慧小霜采纳,获得10
5秒前
情怀应助聪慧小霜采纳,获得30
5秒前
可爱的函函应助聪慧小霜采纳,获得10
5秒前
5秒前
酷波er应助聪慧小霜采纳,获得10
5秒前
无花果应助聪慧小霜采纳,获得30
5秒前
丘比特应助聪慧小霜采纳,获得10
5秒前
乐乐应助聪慧小霜采纳,获得10
5秒前
善学以致用应助聪慧小霜采纳,获得10
5秒前
Jasper应助聪慧小霜采纳,获得30
5秒前
U2完成签到,获得积分10
6秒前
6秒前
smottom应助puzhongjiMiQ采纳,获得10
7秒前
天天快乐应助puzhongjiMiQ采纳,获得10
7秒前
王鹏飞应助puzhongjiMiQ采纳,获得10
7秒前
JamesPei应助puzhongjiMiQ采纳,获得10
7秒前
万能图书馆应助puzhongjiMiQ采纳,获得10
7秒前
王鹏飞应助puzhongjiMiQ采纳,获得10
7秒前
王鹏飞应助puzhongjiMiQ采纳,获得10
7秒前
张童鞋完成签到 ,获得积分10
8秒前
8秒前
Albertxkcj发布了新的文献求助10
10秒前
Jero完成签到 ,获得积分10
10秒前
程程程发布了新的文献求助10
10秒前
KKKKKKK完成签到 ,获得积分10
13秒前
北譩完成签到,获得积分10
15秒前
共享精神应助聪慧小霜采纳,获得10
15秒前
英姑应助聪慧小霜采纳,获得10
15秒前
今后应助聪慧小霜采纳,获得30
15秒前
赘婿应助聪慧小霜采纳,获得10
15秒前
彭于晏应助聪慧小霜采纳,获得10
15秒前
完美世界应助聪慧小霜采纳,获得10
15秒前
卡卡西应助water采纳,获得20
15秒前
大模型应助聪慧小霜采纳,获得50
15秒前
科研通AI2S应助聪慧小霜采纳,获得10
15秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969557
求助须知:如何正确求助?哪些是违规求助? 3514377
关于积分的说明 11173836
捐赠科研通 3249692
什么是DOI,文献DOI怎么找? 1794979
邀请新用户注册赠送积分活动 875537
科研通“疑难数据库(出版商)”最低求助积分说明 804836