荧光素酶
荧光
绿色荧光蛋白
发光
化学
转基因生物
赖氨酸
生物物理学
生物化学
生物
材料科学
转染
基因
光电子学
氨基酸
物理
量子力学
作者
Yuqing Zhang,Yimeng Du,Manjia Li,Dong Zhang,Zheng Xiang,Tao Peng
标识
DOI:10.1002/anie.202001425
摘要
Abstract Formaldehyde (FA) is endogenously produced in living systems through a variety of biological processes and has been implicated in many pathological conditions. Detection tools for biological FA are therefore of great interest. Reported here are novel activity‐based genetically encoded fluorescent and luminescent probes for detecting FA in aqueous solutions and living mammalian cells. A FA‐reactive lysine analogue, PrAK, was site‐specifically incorporated into the essential lysine sites of enhanced green fluorescent protein (EGFP) and firefly luciferase (fLuc) to afford fluorescent and luminescent FA probes, respectively. FA selectively reacts with PrAK residues on EGFP and fLuc through a 2‐aza‐Cope rearrangement, resulting in fluorescence and luminescence turn‐on responses, respectively, to FA selectively over potentially interfering reactive species in aqueous buffer. Moreover, the genetically encoded probes are capable of visualizing FA at physiologically relevant levels in living mammalian cells by fluorescence and luminescence imaging, demonstrating their potential as new tools to explore FA biology.
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