诱导多能干细胞
SOX2
KLF4公司
胚胎干细胞
生物
科斯尔
细胞生物学
再生医学
干细胞
体细胞
转导(生物物理学)
细胞效价
分子生物学
遗传学
基因
生物化学
作者
Xiaodong Liu,Joseph Chen,Jaber Firas,Jacob M. Paynter,Christian M. Nefzger,José M. Polo
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 63-76
被引量:3
标识
DOI:10.1007/978-1-4939-9086-3_5
摘要
Terminally differentiated somatic cells can be reprogrammed into an embryonic stem cell-like state by the forced expression of four transcription factors: Oct4, Klf4, Sox2, and c-Myc (OKSM). These so-called induced pluripotent stem (iPS) cells can give rise to any cell type of the body and thus have tremendous potential for many applications in research and regenerative medicine. Herein, we describe (1) a protocol for the generation of iPS cells from mouse embryonic fibroblasts (MEFs) using a doxycycline (Dox)-inducible lentiviral transduction system; (2) the derivation of clonal iPS cell lines; and (3) the characterization of the pluripotent potential of iPS cell lines using alkaline phosphatase staining, flow cytometry, and the teratoma formation assays.
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