化学
色谱法
分光光度法
酶
分离(微生物学)
生物化学
生物
生物信息学
出处
期刊:Methods in molecular biology
日期:2015-01-01
卷期号:: 41-47
被引量:14
标识
DOI:10.1007/978-1-4939-2694-7_7
摘要
Measuring the concentration of proteins is an essential part of enzyme evaluations or to monitor protein yields during protein isolation procedures. Decisions on the usefulness of any isolation procedure depend on knowing the relative concentrations of a particular protein or enzyme in relation to the concentrations of all the proteins present. Protein concentration in solution is generally measured with spectrophotometry in the UV range or in the presence of dyes or copper interacting with the protein. This review describes protein determination methods for measuring protein concentration in solution.
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