差示扫描量热法
微量滴定板
吞吐量
佐剂
荧光光谱法
重组DNA
化学
抗原
色谱法
荧光
材料科学
生物医学工程
生物
生物化学
计算机科学
免疫学
光学
医学
电信
物理
基因
无线
热力学
作者
Meifeng Nie,Yue Liu,Xiaofen Huang,Zhigang Zhang,Qinjian Zhao
标识
DOI:10.1016/j.xphs.2022.05.015
摘要
Nano/microparticles are widely used as vaccine adjuvants to improve antigen stability and enhance immune response. Conformational stability of a given protein was normally assessed using differential scanning calorimetry (DSC) for the optimization of formulation and for ensuring antigen stability in vaccine products. Here, a higher throughput version, namely the microtiter plate-based differential scanning fluorimetry (DSF) method was developed and optimized for assessing the protein thermal stability in the particulate adjuvant-adsorbed form. Using recombinant human papillomavirus (HPV) vaccine antigens, along with several model proteins, enhanced sensitivity and correlation to the well-established differential scanning calorimetry were demonstrated. Higher throughput and much smaller sample consumption (1/10 ∼ 1/20 of the amount needed as compared to DSC) make the plate-based DSF a method of choice for formulation development, particularly during the early developmental phase of a project where the sample amount is usually quite limited.
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