Transcriptomics integrated with metabolomics reveals underlying mechanisms of cold-induced flesh bleeding in plum (cv. Friar) fruit during storage

肉体 转录组 盐李 代谢组学 氰化物 转录因子 冷库 生物 基因 基因表达 生物化学 植物 细胞生物学 食品科学 园艺 花青素 李子 生物信息学
作者
Ran Xu,Yubei Wang,Zhijun Zhao,Guiqin Qu,Jiankang Cao
出处
期刊:Postharvest Biology and Technology [Elsevier BV]
卷期号:192: 112032-112032 被引量:6
标识
DOI:10.1016/j.postharvbio.2022.112032
摘要

An integrative analysis of the metabolomics and transcriptomics was conducted to reveal the underlying mechanisms responsible for the flesh bleeding of ‘Friar’ plum (Prunus salicina Lindl.) fruit subjected to cold storage. Results from quasi-targeted metabolomics showed that 115 differentially accumulated metabolites were detected and cyanidin derivatives were identified as the main components in red flesh. The accumulation of cyanidin-3-O-glucoside was further quantitatively proved using UPLC-MS. A weighted gene co-correlation network analysis was employed to obtain genes positively associated with flesh bleeding according to the comprehensive transcriptomic profiling, which was verified by Pearson’s correlation coefficient analysis. 21 differentially expressed genes were identified in the biosynthesis of anthocyanins, and 79 overlap up-regulated genes of transcription factors were identified as candidate regulators contributing to flesh bleeding in the fruit in response to cold stress. The up-regulation of genes involving in the MAPK cascade signal system participated in the ethylene signal transduction pathway under cold stress, which stimulates transcription factors and ultimately leads to the activation of structural genes associated with the biosynthesis of anthocyanins. The results of qRT-PCR verified the reliability of transcriptome data. These results would provide a new insight on the mechanism in flesh bleeding in plums during cold storage.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
1秒前
虾仁炒饭发布了新的文献求助10
2秒前
4秒前
橘子海完成签到,获得积分10
4秒前
666发布了新的文献求助10
4秒前
万能图书馆应助搞怪熊猫采纳,获得10
5秒前
虚幻尔阳发布了新的文献求助10
6秒前
呵呵发布了新的文献求助10
6秒前
6秒前
枯蚀完成签到,获得积分10
7秒前
ergrsbf发布了新的文献求助20
8秒前
思源应助大方弘文采纳,获得30
9秒前
杜欣发布了新的文献求助10
9秒前
10秒前
UltraLuo发布了新的文献求助10
10秒前
13秒前
14秒前
玖文完成签到,获得积分10
15秒前
今后应助文献一搜就出采纳,获得10
15秒前
开心采白完成签到 ,获得积分10
15秒前
15秒前
张叮当完成签到,获得积分10
16秒前
16秒前
liu完成签到,获得积分10
18秒前
acffo完成签到 ,获得积分10
19秒前
小雨发布了新的文献求助10
20秒前
lqllll发布了新的文献求助10
20秒前
22秒前
25秒前
风清扬发布了新的文献求助10
27秒前
量子星尘发布了新的文献求助10
28秒前
28秒前
xiaosuda75发布了新的文献求助10
28秒前
28秒前
28秒前
FashionBoy应助小龙虾采纳,获得10
29秒前
JenifferF完成签到,获得积分10
29秒前
大唐元给大唐元的求助进行了留言
29秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969557
求助须知:如何正确求助?哪些是违规求助? 3514377
关于积分的说明 11173836
捐赠科研通 3249692
什么是DOI,文献DOI怎么找? 1794979
邀请新用户注册赠送积分活动 875537
科研通“疑难数据库(出版商)”最低求助积分说明 804836