Hepatic inflammation elicits production of proinflammatory netrin‐1 through exclusive activation of translation

促炎细胞因子 奈特林 炎症 生物 翻译(生物学) 细胞生物学 免疫学 医学 信使核糖核酸 基因 遗传学 轴突引导 轴突
作者
Romain Barnault,Claire Verzeroli,Carole Fournier,Maud Michelet,Anna Rita Redavid,Ievgeniia Chicherova,Marie‐Laure Plissonnier,Annie Adrait,Olga A. Khomich,Fleur Chapus,Mathieu Richaud,Maëva Hervieu,Veronika Reiterer,Federica Grazia Centonze,Julie Lucifora,Birke Bartosch,Michel Rivoire,Hesso Farhan,Yohann Couté,Valbona Mirakaj
出处
期刊:Hepatology [Lippincott Williams & Wilkins]
卷期号:76 (5): 1345-1359 被引量:14
标识
DOI:10.1002/hep.32446
摘要

Abstract Background and Aims Netrin‐1 displays protumoral properties, though the pathological contexts and processes involved in its induction remain understudied. The liver is a major model of inflammation‐associated cancer development, leading to HCC. Approach and Results A panel of cell biology and biochemistry approaches (reverse transcription quantitative polymerase chain reaction, reporter assays, run‐on, polysome fractionation, cross linking immunoprecipitation, filter binding assay, subcellular fractionation, western blotting, immunoprecipitation, stable isotope labeling by amino acids in cell culture) on in vitro–grown primary hepatocytes, human liver cell lines, mouse samples and clinical samples was used. We identify netrin‐1 as a hepatic inflammation‐inducible factor and decipher its mode of activation through an exhaustive eliminative approach. We show that netrin‐1 up‐regulation relies on a hitherto unknown mode of induction, namely its exclusive translational activation. This process includes the transfer of NTN1 (netrin‐1) mRNA to the endoplasmic reticulum and the direct interaction between the Staufen‐1 protein and this transcript as well as netrin‐1 mobilization from its cell‐bound form. Finally, we explore the impact of a phase 2 clinical trial‐tested humanized anti‐netrin‐1 antibody (NP137) in two distinct, toll‐like receptor (TLR) 2/TLR3/TLR6‐dependent, hepatic inflammatory mouse settings. We observe a clear anti‐inflammatory activity indicating the proinflammatory impact of netrin‐1 on several chemokines and Ly6C+ macrophages. Conclusions These results identify netrin‐1 as an inflammation‐inducible factor in the liver through an atypical mechanism as well as its contribution to hepatic inflammation.
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