化学
麦芽糖酶
阿卡波糖
蔗糖酶
α-葡萄糖苷酶
体外
体内
餐后
麦芽糖
色谱法
小檗碱
传统医学
皂甙
蔗糖
药理学
生物化学
生物
糖尿病
医学
酶
生物技术
内分泌学
替代医学
病理
作者
Wenjing Jia,Qimao Bi,Sirong Jiang,Jihong Tao,Liying Liu,Huilan Yue,Xiaohui Zhao
出处
期刊:Food & Function
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:13 (5): 2456-2464
被引量:12
摘要
Codonopsis pilosula (Franch.) Nannf. (CPN), mainly planted in the northwest region, is a traditional Chinese medicine/good health food for nourishing qi and promoting blood circulation. This study firstly evaluated the inhibitory effects of the CPN extraction (CPNE) on α-glucosidase in vitro and in vivo, and tentatively confirmed its chemical ingredients by employing UHPLC-Triple-TOF-MS/MS. The CPNE had strong inhibitory activities against mammalian α-glucosidase (sucrase and maltase) and yeast α-glycosidase with semi-inhibitory concentrations (IC50) of 0.241 mg mL-1, 0.326 mg mL-1 and 1.167 mg mL-1, respectively. In addition, the CPNE could significantly decrease the postprandial blood glucose (PBG) levels in the sucrose/maltose/starch tolerance assays of diabetic mice. Furthermore, a total of 29 compounds, including 3 alkaloids, 13 phenolic acids, 8 alcohol glycosides and 5 alkynosides, were assigned based on comparison with the standards and references, as well as the analysis of main fragments. These results demonstrated that CPN could be used as an adjuvant therapy or dietary supplements to effectively control the occurrence and development of diabetes.
科研通智能强力驱动
Strongly Powered by AbleSci AI