Definition of specific peptide motifs for four major HLA-A alleles.

氨基酸 肽序列 生物 表位 人类白细胞抗原 序列母题 结构母题 生物化学 遗传学 化学 基因 抗原
作者
Ralph T. Kubo,Alessandro Sette,Howard M. Grey,Ettore Appella,Kazuyasu Sakaguchi,Nannan Zhu,David Arnott,Nicholas E. Sherman,Jeffrey Shabanowitz,Hanspeter Michel
出处
期刊:Journal of Immunology [American Association of Immunologists]
卷期号:152 (8): 3913-3924 被引量:267
标识
DOI:10.4049/jimmunol.152.8.3913
摘要

Allele-specific motifs for the human MHC class I molecules, HLA-A1, A3, A11, and A24 were characterized by three complementary approaches. First, amino acid sequence analysis of acid eluted peptide pools from affinity purified class I molecules defined putative motifs 9 or 10 amino acids in length and bearing critical anchor residues at position 2 and at the COOH-terminal. These motifs were distinct, with the exception of the HLA-A3 and A11 motifs that were very similar to each other. Second, the correctness of these putative motifs was verified by analyzing the binding capacity of polyalanine peptide analogues to purified HLA-A molecules. Several alternative anchor residues that were not obvious from the pooled peptide sequencing analysis were identified. Third, sequences of individual peptides eluted from HLA-A1, A11, and A24 were determined by tandem mass spectrometry. Nonamers were the predominant species, although peptides of 8, 10, 11, and 12 amino acids in length were also identified. These peptides displayed anchor residues predicted by the specific motifs at position 2 and at the COOH-terminal, regardless of peptide length. Synthetic versions of the naturally processed peptides were shown to bind to the appropriate HLA-A alleles with IC50 values in the 0.3- to 200-nM range. A rational approach to search Ags with known amino acid sequences for epitopes restricted by some of the most common HLA-A types and of potential clinical importance is now feasible.
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