INTESTINAL MICROFLORA OF KUTUM RUTILUS FRISII KUTUM UNDER DIETARY SUPPLEMENTATION WITH PROBIOTIC AND VITAMIN C

GHOMI, M. R., Z. HESHMATIPOUR, R. M. NAZARI, M. SOHRABNEJAD, M. ZAREI, M. NIKOO, M. OVISSIPOUR and A. ESMAEILI MOLLA, 2010. Intestinal microflora of kutum Rutilus frisii kutum under dietary supplementation with probiotic and vitamin C. Bulg. J. Agric. Sci., 16: 635-642 The purpose of this study was to evaluate the effect of dietary probiotic and vitamin C on intestinal microflora of kutum Rutilus frisii kutum juveniles. The juveniles were fed to apparent satiation the diet supplemented with probiotic Primalac® (0, 0.1 and 0.3%), vitamin C (0, 200 and 400 mg kg-1) or their combination for 8 weeks. Result indicated that fish fed with the control diets without supplementation exhibited statistically (P<0.05) higher mortality than other treatments. On the basis of carbohydrate utilization profiles using API 50 CHL kits (fermentation pattern), the intestinal detected strains were divided into the three groups (Lactobacillus brevis, L. plantarum, and L. lactis). With increasing the culture period, number of total bacterial counts and lactic acid bacteria increased.