亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Towards simultaneous individual and tissue identification: A proof-of-principle study on parallel sequencing of STRs, amelogenin, and mRNAs with the Ion Torrent PGM

釉原蛋白 离子半导体测序 生物 计算生物学 法医鉴定 遗传学 大规模并行测序 聚合酶链反应 基因组DNA 分子生物学 DNA测序 DNA 基因
作者
Dmtry Zubakov,Iris Kokmeijer,Arwin Ralf,Narasimhan Rajagopalan,Lisa M. Calandro,Sharon Wootton,Reina Langit,Chien‐Wei Chang,Robert Lagacé,Manfred Kayser
出处
期刊:Forensic Science International-genetics [Elsevier BV]
卷期号:17: 122-128 被引量:37
标识
DOI:10.1016/j.fsigen.2015.04.002
摘要

DNA-based individual identification and RNA-based tissue identification represent two commonly-used tools in forensic investigation, aiming to identify crime scene sample donors and helping to provide links between DNA-identified sample donors and criminal acts. Currently however, both analyses are typically performed separately. In this proof-of-principle study, we developed an approach for the simultaneous analysis of forensic STRs, amelogenin, and forensic mRNAs based on parallel targeted DNA/RNA sequencing using the Ion Torrent Personal Genome Machine(®) (PGM™) System coupled with the AmpliSeq™ targeted amplification. We demonstrated that 9 autosomal STRs commonly used for individual identification (CSF1PO, D16S539, D3S1358, D5S818, D7S820, D8S1179, TH01, TPOX, and vWA), the AMELX/AMELY system widely applied for sex identification, and 12 mRNA markers previously established for forensic tissue identification (ALAS2 and SPTB for peripheral blood, MMP10 and MMP11 for menstrual blood, HTN3 and STATH for saliva, PRM1 and TGM4 for semen, CYP2B7P1 and MUC4 for vaginal secretion, CCL27 and LCE1C for skin) together with two candidate reference mRNA markers (HPRT1 and SDHA) can all be successfully combined. Unambiguous mRNA-based tissue identification was achieved in all samples from all forensically relevant tissues tested, and STR sequencing analysis of the tissue sample donors was 100% concordant with conventional STR profiling using a commercial kit. Successful STR analysis was obtained from 1ng of genomic DNA and mRNA analysis from 10ng total RNA; however, sensitivity limits were not investigated in this proof-of-principle study and are expected to be much lower. Since dried materials with noticeable RNA degradation and small DNA/RNA amplicons with high-coverage sequencing were used, the achieved correct individual and tissue identification demonstrates the suitability of this approach for analyzing degraded materials in future forensic applications. Overall, our study demonstrates the feasibility of simultaneously obtaining multilocus STR, amelogenin, and multilocus mRNA information for combined individual and tissue identification from a small sample of degraded biological material. Moreover, our study marks the first step towards combining many DNA/RNA markers for various forensic purposes to increase the effectiveness of molecular forensic analysis and to allow more forensically relevant information to be obtained from limited forensic material.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
3秒前
4秒前
李李发布了新的文献求助10
5秒前
bksqc发布了新的文献求助10
7秒前
要文献啊完成签到 ,获得积分10
7秒前
12秒前
WUHUIWEN完成签到,获得积分10
16秒前
Liiiiiiiiii发布了新的文献求助10
16秒前
17秒前
20秒前
尊敬夜南发布了新的文献求助10
22秒前
韦雪莲完成签到 ,获得积分10
39秒前
wz完成签到,获得积分10
40秒前
46秒前
Liiiiiiiiii完成签到,获得积分10
49秒前
杜德爽发布了新的文献求助10
51秒前
好好搞科研完成签到 ,获得积分10
53秒前
54秒前
热情的寄瑶完成签到 ,获得积分10
56秒前
yanglinhai完成签到 ,获得积分10
1分钟前
饱满含玉完成签到,获得积分10
1分钟前
1分钟前
1分钟前
CipherSage应助bksqc采纳,获得10
1分钟前
1分钟前
大神完成签到,获得积分0
1分钟前
爆米花应助科研通管家采纳,获得10
1分钟前
研友_VZG7GZ应助科研通管家采纳,获得10
1分钟前
1分钟前
1分钟前
1分钟前
2分钟前
科研通AI2S应助PEITON采纳,获得10
2分钟前
bksqc发布了新的文献求助10
2分钟前
Qiuyajing完成签到,获得积分10
2分钟前
JOJO完成签到,获得积分10
2分钟前
3分钟前
深情安青应助干净涵梅采纳,获得10
3分钟前
沉静的安青完成签到 ,获得积分10
3分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990031
求助须知:如何正确求助?哪些是违规求助? 3532108
关于积分的说明 11256334
捐赠科研通 3270943
什么是DOI,文献DOI怎么找? 1805146
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228