串扰
染色质免疫沉淀
增强子
转录因子
细胞生物学
计算生物学
抄写(语言学)
生物
基因
发起人
遗传学
电子工程
基因表达
工程类
语言学
哲学
作者
Dilem Shakir,Michael Batie,Sónia Rocha
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 255-265
被引量:1
标识
DOI:10.1007/978-1-0716-1669-7_15
摘要
Hypoxia and inflammation are intensely connected in a functional crosstalk. Within this crosstalk, two major transcription factors take center stage: HIF and NF-κB. To investigate transcription factor function, an important aspect is its ability to bind DNA. The most appropriate method to study this property in cells is the use of chromatin immunoprecipitation followed by qPCR and/or next generation sequencing. This allows identification of potentially directly regulated genes as well as enhancer regions. Here we describe the ChIP-qPCR method in detail, including key aspects important for the success of the technique.
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