Quantitative analysis of binary mixtures of entecavir using solid-state analytical techniques with chemometric methods

化学 恩替卡韦 检出限 分析化学(期刊) 二进制数 傅里叶变换红外光谱 色谱法 乙型肝炎病毒 数学 物理 病毒 病毒学 量子力学 拉米夫定 生物 算术
作者
Mingdi Liu,Peng Shi,Guangle Wang,Gang Wang,Ping Song,Yumin Liu,Songgu Wu,Junbo Gong
出处
期刊:Arabian Journal of Chemistry [Elsevier BV]
卷期号:14 (10): 103360-103360 被引量:5
标识
DOI:10.1016/j.arabjc.2021.103360
摘要

Entecavir (ETV), a guanosine analogue, can be rapidly phosphorylated to the active intracellular triphosphate form that inhibits replication of the hepatitis B virus (HBV). This work aims to develop useful methods to quantify the content of ETV-A in ETV-H and ETV-A binary mixtures. The integral enthalpy value of the phase transformation in the DSC thermogram was selected to quantify the content of ETV-A in binary mixture. Different ranges (full spectrum and partial spectrum) of the PXRD and ATR-FTIR data versus ETV-A content were used to develop partial least squares (PLS) calibration curves with different pre-processing algorithms including multiplicative scatter correction (MSC), standard normal variate (SNV), wavelet transform (WT) or their combinations. The standard curve obtained for the DSC technique is Y = 100.455 + 7.255X, R2 = 0.997, in the ETV-A contents range of 0–100% w/w% in binary mixtures. Limit of detection (LOD) of ETV-A was 0.909% and limit of quantitation (LOQ) is 2.755%. The best correction models for PXRD and ATR-FTIR techniques, in the ETV-A contents range of 0–100% w/w% in binary mixtures, were Y = 0.346 + 0.999X, R2 = 0.999 in the 2θ range of 4-6° with 14-34° and Y = 0.767 + 0.991X, R2 = 0.991 in the range of 3500–2500 cm−1 with 2000–400 cm−1 pretreated by MSC + WT, respectively. Limit of detection (LOD) of ETV-A was 1.394% and limit of quantitation (LOQ) was 4.224% for PXRD technique. LOD and LOQ for ATR-FTIR technique were 9.124% and 27.648%. The results show that DSC, PXRD and ATR-FTIR techniques are precise and accurate, and could be used for the measurement of ETV-A content in the binary mixtures.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
SciGPT应助臭小子采纳,获得10
刚刚
Miya_han发布了新的文献求助10
1秒前
kassidy发布了新的文献求助10
2秒前
3秒前
Owen应助王晓梅采纳,获得10
3秒前
Orange应助学习通采纳,获得10
4秒前
5秒前
Singularity应助专注乌冬面采纳,获得10
5秒前
yznfly应助小徐采纳,获得20
5秒前
量子星尘发布了新的文献求助10
6秒前
北风发布了新的文献求助10
6秒前
球球完成签到,获得积分10
7秒前
8秒前
她与星辰皆失完成签到 ,获得积分10
9秒前
小本本发布了新的文献求助10
10秒前
12秒前
健忘曼冬发布了新的文献求助10
13秒前
猪猪发布了新的文献求助30
14秒前
大模型应助无限映波采纳,获得10
14秒前
14秒前
15秒前
16秒前
桐桐应助grace采纳,获得10
17秒前
17秒前
诺贝尔候选人完成签到 ,获得积分10
18秒前
556应助仲达采纳,获得10
18秒前
boshi发布了新的文献求助10
18秒前
小菜发布了新的文献求助10
19秒前
北风完成签到,获得积分10
22秒前
Serena510完成签到 ,获得积分10
22秒前
慕青应助欣慰的乌冬面采纳,获得10
23秒前
ding应助方仔采纳,获得10
24秒前
hhhh应助bliss采纳,获得20
24秒前
25秒前
大个应助苗条的辣辣采纳,获得10
25秒前
猪猪完成签到,获得积分10
26秒前
26秒前
研友_ngkEgn完成签到,获得积分10
26秒前
赘婿应助小菜采纳,获得10
26秒前
完美世界应助shinn采纳,获得30
28秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975339
求助须知:如何正确求助?哪些是违规求助? 3519670
关于积分的说明 11199199
捐赠科研通 3256002
什么是DOI,文献DOI怎么找? 1798043
邀请新用户注册赠送积分活动 877386
科研通“疑难数据库(出版商)”最低求助积分说明 806305