PLGA公司
免疫系统
免疫原性
树突状细胞
化学
抗原
溶解
免疫疗法
外周血单个核细胞
癌细胞
癌症研究
癌症免疫疗法
癌症
体外
免疫学
医学
生物化学
内科学
作者
Cristiana Berti,Michele Graciotti,Alice Boarino,Chakradhar Yakkala,Lana E. Kandalaft,Harm‐Anton Klok
标识
DOI:10.1002/mabi.202100356
摘要
Cancer vaccination is a powerful strategy to combat cancer. A very attractive approach to prime the immune system against cancer cells involves the use of tumor lysate as antigen source. The immunogenicity of tumor lysate can be further enhanced by treatment with hypochlorous acid. This study explores poly(lactic-co-glycolic acid) (PLGA) nanoparticles to enhance the delivery of oxidized tumor lysate to dendritic cells. Using human donor-derived dendritic cells, it is found that the use of PLGA nanoparticles enhances antigen uptake and dendritic cell maturation, as compared to the use of the free tumor lysate. The ability of the activated dendritic cells to stimulate autologous peripheral blood mononuclear cells (PBMCs) is assessed in vitro by coculturing PBMCs with A375 melanoma cells. Live cell imaging analysis of this experiment highlights the potential of nanoparticle-mediated dendritic-cell-based vaccination approaches. Finally, the efficacy of the PLGA nanoparticle formulation is evaluated in vivo in a therapeutic vaccination study using B16F10 tumor-bearing C57BL/6J mice. Animals that are challenged with the polymer nanoparticle-based oxidized tumor lysate formulation survive for up to 50 days, in contrast to a maximum of 41 days for the group that receives the corresponding free oxidized tumor lysate-based vaccine.
科研通智能强力驱动
Strongly Powered by AbleSci AI