Sarcodon aspratus Extract Ameliorates Dextran Sulfate Sodium‐Induced Colitis in Mouse Colon and Mesenteric Lymph Nodes

Abstract Mushrooms have been previously investigated for their immune‐modulating and anti‐inflammatory properties. We examined whether the anti‐inflammatory properties of Sarcodon aspratus ethanol extract (SAE) could elicit protective effects against dextran sulfate sodium (DSS)‐induced colitis in vivo . Male C57/BL6 mice were randomly assigned to 1 of 4 treatment groups: control (CON; n = 8), DSS‐treated (DSS; n = 9), DSS+SAE at 50 mg/kg BW (SAE50; n = 8), and DSS+SAE at 200 mg/kg BW groups (SAE200; n = 9). DSS treatment induced significant weight loss, which was significantly recovered by SAE200. Although SAE did not affect DSS‐mediated reductions in colon length, it improved diarrhea and rectal bleeding induced by DSS. SAE at 200 mg/kg BW significantly attenuated IL‐6 and enhanced IL‐10 expression in mesenteric lymph nodes (MLN), and significantly reduced IL‐6 levels in splenocytes. SAE200 also significantly attenuated DSS‐induced increase in IL‐6 and IL‐1β, and reductions in IL‐10 in colon tissue. High levels of SAE were also observed to significantly decrease inflammatory COX‐2 expression that was upregulated by DSS in mice colon. These findings may have relevance for novel therapeutic strategies to mitigate inflammatory bowel disease‐relevant inflammatory responses, via the direct and indirect anti‐inflammatory activity of SAE. We also found that SAE harbors significant quantities of total fiber and β‐glucan, suggesting a possible role for these components in protection against DSS‐mediated colitis.