Gibel carp Carassius auratus gut microbiota after oral administration of trimethoprim/ sulfamethoxazole

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 99:207-213 (2012) - DOI: https://doi.org/10.3354/dao02477 Gibel carp Carassius auratus gut microbiota after oral administration of trimethoprim/ sulfamethoxazole Yuchun Liu1, Zhigang Zhou1,*, Ning Wu2, Yu Tao2, Li Xu1, Yanan Cao1, Yuting Zhang1, Bin Yao1 1Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China 2College of Life Science and Technology, Beijing Forestry University, Beijing 100083, PR China *Corresponding author. Email: zhouzhigang@caas.net.cn ABSTRACT: Trimethoprim/sulfamethoxazole is widely used in the treatment of infectious diseases caused by bacterial pathogens in aquaculture. However, the practice of antibiotic administration can promote the emergence of resistant strains of bacteria and result in a wane in efficacy over time. The objective of this study was to assess the effect of oral treatment with trimethoprim/sulfamethoxazole on the gastrointestinal (GI) microbiota of healthy gibel carp and those affected with bacterial enteritis. By using denaturing gradient gel electrophoresis (DGGE), the changes in the predominant bacterial communities were directly depicted for the first time. The main findings were (1) Actinobacteria, Firmicutes and Proteobacteria were the predominant phyla in the healthy gibel carp intestine; (2) administration of antibiotics had a more profound impact on the intestinal microflora of healthy fish than of the diseased ones; and (3) Enterobacteriaceae might be one of the major drug-resistant bacteria in the gibel carp intestine. This study provides an insight into the effect of antibiotic treatment on the establishment and colonization of fish GI microbiota and speculates on some possible drug-resistant bacteria. KEY WORDS: Gastrointestinal microbiota · Gibel carp · Trimethoprim/sulfamethoxazole · PCR−DGGE Full text in pdf format PreviousNextCite this article as: Liu Y, Zhou Z, Wu N, Tao Y and others (2012) Gibel carp Carassius auratus gut microbiota after oral administration of trimethoprim/ sulfamethoxazole. Dis Aquat Org 99:207-213. https://doi.org/10.3354/dao02477 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 99, No. 3. Online publication date: July 25, 2012 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2012 Inter-Research.