Analysis of the proteomic profile of chronic pressure ulcers

化学 角蛋白 糖基化 蛋白质亚单位 伤口愈合 分子生物学 受体 生物化学 病理 生物 免疫学 医学 基因
作者
Laura E. Edsberg,Jennifer T. Wyffels,Michael S. Brogan,Kristin M. Fries
出处
期刊:Wound Repair and Regeneration [Wiley]
卷期号:20 (3): 378-401 被引量:60
标识
DOI:10.1111/j.1524-475x.2012.00791.x
摘要

Abstract Analysis of the proteomic profile of pressure ulcers over time is a critical step in the identification of biomarkers of healing or nonhealing in pressure ulcers. The wound fluid from 32 subjects with 42 pressure ulcers was evaluated over 6 weeks at 15 time points. Samples specific to both the interior and the periphery of the wound bed were collected. Antibody screening arrays, isobaric tags for relative and absolute quantitation with mass spectrometry and multiplexed microarrays were used to characterize wound fluid and results were correlated with clinical outcome. Twenty‐one proteins were found to distinguish between healed and chronic wounds and 19 proteins were differentially expressed between the interior and periphery of wounds. Four proteins, pyruvate kinase isozymes M 1/ M 2, profilin‐1, Ig lambda‐1 chain C regions, and Ig gamma‐1 chain C region, were present in lower levels for periphery samples when compared to interior samples and six proteins, keratin, type II cytoskeletal 6 A ( KRT6A ), keratin, type I cytoskeletal 14, S 100 calcium binding proteins A 7, alpha‐1‐antitrypsin precursor, hemoglobin subunit alpha, and hemoglobin subunit beta, were present in higher levels in periphery samples when compared with interior samples. S 100 calcium binding protein A 6, S 100 calcium binding protein A 7, and soluble receptor for advanced glycation end‐products had higher levels in the periphery of chronic wounds vs. the interior in planar arrays. A significant temporal trend was noted for monokine induced by gamma interferon (MIG), synonomous with chemokine (C‐X‐C motif) ligand 9 (CXCL9), which increased as wounds healed and remained nearly constant for ulcers that were not approaching closure.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
所所应助罗拉采纳,获得10
2秒前
2秒前
天天快乐应助忐忑的阑香采纳,获得10
4秒前
哈哈完成签到,获得积分10
4秒前
苏su关注了科研通微信公众号
4秒前
少主发布了新的文献求助10
4秒前
5秒前
5秒前
6秒前
安静发布了新的文献求助10
7秒前
上官若男应助PANYIAO采纳,获得10
8秒前
11秒前
tingting9发布了新的文献求助10
11秒前
11秒前
12秒前
12秒前
Akim应助高手采纳,获得10
12秒前
12秒前
李健的小迷弟应助ZZZ采纳,获得10
12秒前
你好呀嘻嘻完成签到 ,获得积分10
12秒前
zwxzghgz完成签到,获得积分10
13秒前
hello发布了新的文献求助10
17秒前
17秒前
17秒前
17秒前
crazy完成签到,获得积分10
18秒前
咩咩羊发布了新的文献求助10
19秒前
易儿发布了新的文献求助10
19秒前
PANYIAO发布了新的文献求助10
20秒前
李彪完成签到,获得积分20
20秒前
小柒完成签到,获得积分10
21秒前
Lucas应助13223456采纳,获得10
22秒前
24秒前
chris发布了新的文献求助50
24秒前
简单面包完成签到,获得积分10
27秒前
踏实的老四完成签到,获得积分20
27秒前
29秒前
洪汉发布了新的文献求助50
31秒前
32秒前
33秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989242
求助须知:如何正确求助?哪些是违规求助? 3531393
关于积分的说明 11253753
捐赠科研通 3270010
什么是DOI,文献DOI怎么找? 1804868
邀请新用户注册赠送积分活动 882084
科研通“疑难数据库(出版商)”最低求助积分说明 809136