枯草芽孢杆菌
地衣芽孢杆菌
计算生物学
核糖体结合位点
基因
苏云金杆菌
生物
翻译(生物学)
基因表达
合成生物学
芽孢杆菌(形态)
生物系统
遗传学
计算机科学
信使核糖核酸
细菌
作者
Xiaolan Rao,Dian Li,Zhaowei Su,Christopher T. Nomura,Shouwen Chen,Qin Wang
标识
DOI:10.1016/j.ymben.2023.11.002
摘要
Bacillus species, such as Bacillus subtilis and Bacillus licheniformis, are important industrial bacteria. However, there is a lack of standardized and predictable genetic tools for convenient and reproducible assembly of genetic modules in Bacillus species to realize their full potential. In this study, we constructed a Ribosome Binding Site (RBS) library in B. licheniformis, which provides incremental regulation of expression levels over a 104-fold range. Additionally, we developed a model to quantify the resulting translation rates. We successfully demonstrated the robust expression of various target genes using the RBS library and showed that the model accurately predicts the translation rates of arbitrary coding genes. Importantly, we also extended the use of the RBS library and prediction model to B. subtilis, B. thuringiensis, and B. amyloliquefacie. The versatility of the RBS library and its prediction model enables quantification of biological behavior, facilitating reliable forward engineering of gene expression.
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