亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Gelatin-based 3D biomimetic scaffolds platform potentiates culture of cancer stem cells in esophageal squamous cell carcinoma

癌症干细胞 细胞外基质 肿瘤微环境 癌症研究 干细胞 细胞培养 细胞生物学 癌细胞 癌变 材料科学 癌症 生物 肿瘤细胞 遗传学
作者
Yenan Wu,Haiwei Liang,Aiping Luo,Yong Li,Zhiqiang Liu,Xin Li,Wenxin Li,Kaini Liang,Junyang Li,Zhihua Liu,Yanan Du
出处
期刊:Biomaterials [Elsevier]
卷期号:302: 122323-122323 被引量:7
标识
DOI:10.1016/j.biomaterials.2023.122323
摘要

Cancer stem cells (CSCs) are crucial for tumorigenesis, metastasis, and therapy resistance in esophageal squamous cell carcinoma (ESCC). To further elucidate the mechanism underlying characteristics of CSCs and develop CSCs-targeted therapy, an efficient culture system that could expand and maintain CSCs is needed. CSCs reside in a complex tumor microenvironment, and three-dimensional (3D) culture systems of biomimetic scaffolds are expected to better support the growth of CSCs by recapitulating the biophysical properties of the extracellular matrix (ECM). Here, we established gelatin-based 3D biomimetic scaffolds mimicking the stiffness and collagen content of ESCC, which could enrich ESCC CSCs efficiently. Biological changes of ESCC cells laden in scaffolds with three different viscoelasticity emulating physiological stiffness of esophageal tissues were thoroughly investigated in varied aspects such as cell morphology, viability, cell phenotype markers, and transcriptomic profiling. The results demonstrated the priming effects of viscoelasticity on the stemness of ESCC. The highly viscous scaffolds (G': 6-403 Pa; G'': 2-75 Pa) better supported the enrichment of ESCC CSCs, and the TGF-beta signaling pathway might be involved in regulating the stemness of ESCC cells. Compared to two-dimensional (2D) cultures, highly viscous scaffolds significantly promoted the clonal expansion of ESCC cells in vitro and tumor formation ability in vivo. Our findings highlight the crucial role of biomaterials' viscoelasticity for the 3D culture of ESCC CSCs in vitro, and this newly-established culture system represents a valuable platform to support their growth, which could facilitate the CSCs-targeted therapy in the future.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
舒心远侵发布了新的文献求助10
5秒前
可爱的函函应助舒心远侵采纳,获得10
26秒前
zoie0809完成签到,获得积分10
50秒前
Emperor完成签到 ,获得积分0
56秒前
舒心远侵完成签到,获得积分10
1分钟前
天天快乐应助高小羊采纳,获得10
3分钟前
3分钟前
高小羊发布了新的文献求助10
3分钟前
打打应助郜南烟采纳,获得10
3分钟前
高小羊完成签到,获得积分10
3分钟前
LouieHuang完成签到,获得积分20
4分钟前
4分钟前
郜南烟发布了新的文献求助10
4分钟前
wanci应助郜南烟采纳,获得10
4分钟前
上官若男应助zhangyimg采纳,获得10
4分钟前
5分钟前
Lorin完成签到 ,获得积分10
5分钟前
5分钟前
zhangyimg发布了新的文献求助10
5分钟前
科目三应助zhangyimg采纳,获得10
5分钟前
gszy1975完成签到,获得积分10
6分钟前
圆圆的波仔发布了新的文献求助100
6分钟前
JamesPei应助科研通管家采纳,获得10
7分钟前
郗妫完成签到,获得积分10
7分钟前
8分钟前
郜南烟发布了新的文献求助10
8分钟前
Venus完成签到 ,获得积分10
9分钟前
在水一方应助chenyuns采纳,获得30
10分钟前
JACk完成签到 ,获得积分10
10分钟前
10分钟前
chenyuns发布了新的文献求助30
10分钟前
爱静静应助李伟采纳,获得10
10分钟前
10分钟前
zhangyimg发布了新的文献求助10
11分钟前
11分钟前
郜南烟发布了新的文献求助10
11分钟前
斯文败类应助郜南烟采纳,获得10
11分钟前
思源应助chenyuns采纳,获得20
11分钟前
Akim应助chenyuns采纳,获得20
12分钟前
领导范儿应助圆圆的波仔采纳,获得10
13分钟前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
叶剑英与华南分局档案史料 500
Foreign Policy of the French Second Empire: A Bibliography 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3146771
求助须知:如何正确求助?哪些是违规求助? 2798063
关于积分的说明 7826621
捐赠科研通 2454573
什么是DOI,文献DOI怎么找? 1306394
科研通“疑难数据库(出版商)”最低求助积分说明 627708
版权声明 601527