谷氨酸棒杆菌
代谢工程
氨基酸
生物化学
天冬氨酸
代谢途径
赖氨酸
合成代谢
苏氨酸
氨基酸合成
异亮氨酸
生物合成
蛋氨酸
生物
发酵
化学
新陈代谢
亮氨酸
丝氨酸
酶
基因
作者
Yusheng Wang,Bai Yunlong,Qi Zeng,Zeyuan Jiang,Brian Liu,Xiyan Wang,Xiaoting Liu,Chunlei Liu,Weihong Min
标识
DOI:10.1016/j.ijbiomac.2023.126916
摘要
L-aspartic acid, L-threonine, L-isoleucine, l-lysine, and L-methionine constitute the l-aspartate amino acids (AFAAs). Except for L-aspartic acid, these are essential amino acids that cannot be synthesized by humans or animals themselves. E. coli and C. glutamicum are the main model organisms for AFAA production. It is necessary to reconstitute microbial cell factories and the physiological state of industrial fermentation cells for in-depth research into strains with higher AFAA production levels and optimal growth states. Considering that the anabolic pathways of the AFAAs and engineering modifications have rarely been reviewed in the latest progress, this work reviews the central metabolic pathways of two strains and strategies for the metabolic engineering of AFAA synthetic pathways. The challenges posed by microbial physiology in AFAA production and possible strategies to address them, as well as future research directions for constructing strains with high AFAA production levels, are discussed in this review article.
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