清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

In vitro anti-bactrical activity and its preliminary mechanism of action of the non-medicinal parts of Sanguisorba officinalis L. against Helicobacter pylori infection

肉汤微量稀释 传统医学 药用植物 官房 幽门螺杆菌 最小抑制浓度 抗菌剂 抗菌活性 植物化学 化学 微生物学 生物 医学 细菌 遗传学
作者
Pengting Chen,Meiyun Chen,Peng Chang,Jiahui Yan,Xue Shen,Weijia Zhang,Y. Yuan,Gin Gin Gan,Xiaojun Luo,Weichun Zhu,Meicun Yao
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:318: 116981-116981 被引量:8
标识
DOI:10.1016/j.jep.2023.116981
摘要

Sanguisorba officinalis L. (S. officinalis L.), known as Di Yu (DY) in Traditional Chinese Medicine (TCM), are used to treat burns, vomiting of blood, asthma, intestinal infections, and dermatitis. It has been reported that the root of DY has a significant inhibitory effect on Helicobacter pylori (H. pylori). However, there is currently little research on the composition analysis and anti-H. pylori infection properties of the non-medicinal parts of DY, such as its stems, leaves, and flowers.The commonly used eradication therapies for H. pylori infection are antibiotic-based therapies. With the increasing antibiotic resistance of H. pylori, it is urgent to find effective alternative therapies. To find alternative therapies and increase the utilization of DY, this study aims to investigate the phytochemistry profile, in vitro anti-H. pylori activity, and preliminary antibacterial mechanism of the non-medicinal parts of DY.The non-medicinal parts of DY extracts were obtained by using hot water reflux method. The chemical composition of these extracts was analyzed using colorimetric method, high-performance liquid chromatography (HPLC), and ultra-high-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS). The in vitro anti-H. pylori activity was investigated using broth microdilution method, checkerboard dilution method, time-kill curve, time-inhibition curve, scanning electron microscopy, and transmission electron microscopy. Transcriptional sequencing technology was used to study the effect of DY stems and flowers on the gene expression of H. pylori and explore possible antibacterial mechanisms.The non-medicinal parts of DY contain abundant phytochemicals, such as total phenols and total flavonoids, and possess strong inhibitory and bactericidal activity against both standard and clinical strains of H. pylori in vitro. The MIC was 80-1280 μg/mL and the MBC was 80-2560 μg/mL, and the strength of the antibacterial effects was dependent on the concentration of phytochemicals (total polyphenols, gallic acid and ellagic acid). In addition, the combination of non-medicinal parts of DY with antibiotics, such as amoxicillin, metronidazole, levofloxacin, and clarithromycin, did not result in any antagonistic effects. All of them could disrupt the morphology, internal microscopic and cell wall structures of H. pylori thereby acting as an inhibitor. The mechanism of action was found to be the disruption of H. pylori morphology, internal microstructure, and cell wall. Transcriptomic analysis showed that the non-medicinal parts of DY significantly regulated the gene expression of H. pylori, especially the metabolic pathway.This study analyzed the chemical composition of the non-medicinal parts of DY and confirmed its inhibitory and bactericidal activities against H. pylori, both standard and clinical strains. Additional, the mechanism of inhibition involves disrupting the structure of H. pylori cells, altering gene expression, and interfering with bacterial metabolic pathways. This study provides a reference for further resource utilization and the development of H. pylori drugs using the non-medicinal parts of DY.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
ktw完成签到,获得积分10
2秒前
kmzzy完成签到,获得积分10
37秒前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
2分钟前
3分钟前
一条摆摆的沙丁鱼完成签到 ,获得积分10
3分钟前
3分钟前
yyf发布了新的文献求助10
3分钟前
claud完成签到 ,获得积分0
3分钟前
yyf完成签到,获得积分10
3分钟前
ffdhdh完成签到,获得积分10
4分钟前
白天科室黑奴and晚上实验室牛马完成签到 ,获得积分10
5分钟前
5分钟前
Akim应助亦风采纳,获得10
5分钟前
5分钟前
亦风发布了新的文献求助10
5分钟前
情怀应助刘紫媛采纳,获得10
5分钟前
科研通AI2S应助亦风采纳,获得10
5分钟前
亦风完成签到,获得积分10
6分钟前
6分钟前
偷得浮生半日闲完成签到 ,获得积分10
6分钟前
jintian完成签到 ,获得积分10
6分钟前
7分钟前
fufufu123完成签到 ,获得积分10
7分钟前
桐桐应助科研通管家采纳,获得10
7分钟前
puzhongjiMiQ完成签到,获得积分10
7分钟前
8分钟前
李健的粉丝团团长应助lzzz采纳,获得10
9分钟前
9分钟前
刘紫媛发布了新的文献求助10
9分钟前
Raul完成签到 ,获得积分10
10分钟前
lql完成签到 ,获得积分10
10分钟前
可爱的函函应助鲅鱼圈采纳,获得10
10分钟前
10分钟前
lzzz发布了新的文献求助10
11分钟前
英喆完成签到 ,获得积分10
11分钟前
我是老大应助畅快的毛衣采纳,获得10
11分钟前
11分钟前
11分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990469
求助须知:如何正确求助?哪些是违规求助? 3532166
关于积分的说明 11256513
捐赠科研通 3271046
什么是DOI,文献DOI怎么找? 1805207
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234