清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Puerarin alleviated oxidative stress and ferroptosis during renal fibrosis induced by ischemia/reperfusion injury via TLR4/Nox4 pathway in rats

葛根素 氮氧化物4 天狼星红 氧化应激 丙二醛 纤维化 化学 免疫印迹 内分泌学 内科学 药理学 医学 NADPH氧化酶 病理 生物化学 替代医学 基因
作者
Jun Jian,Dan Wang,Yufeng Xiong,Jingsong Wang,Qingyuan Zheng,Zhengyu Jiang,Jiacheng Zhong,Song Yang,Lei Wang
出处
期刊:Acta Cirurgica Brasileira [SciELO]
卷期号:38 被引量:13
标识
DOI:10.1590/acb382523
摘要

Purpose: To investigate the role of puerarin on renal fibrosis and the underlying mechanism in renal ischemia and reperfusion (I/R) model. Methods: Rats were intraperitoneally injected with puerarin (50 or 100 mg/kg) per day for one week before renal I/R. The level of renal collagen deposition and interstitial fibrosis were observed by hematoxylin and eosin and Sirius Red staining, and the expression of α-smooth muscle actin (α-SMA) was examined by immunohistochemical staining. The ferroptosis related factors and TLR4/Nox4-pathway-associated proteins were detected by Western blotting. Results: Puerarin was observed to alleviate renal collagen deposition, interstitial fibrosis and the α-SMA expression induced by I/R. Superoxide dismutase (SOD) activities and glutathione (GSH) level were decreased in I/R and hypoxia/reoxygenation (H/R), whereas malondialdehyde (MDA) and Fe2+ level increased. However, puerarin reversed SOD, MDA, GSH and Fe2+ level changes induced by I/R and H/R. Besides, Western blot indicated that puerarin inhibited the expression of ferroptosis related factors in a dose-dependent manner, which further demonstrated that puerarin had the effect to attenuate ferroptosis. Moreover, the increased expression of TLR/Nox4-pathway-associated proteins were observed in I/R and H/R group, but puerarin alleviated the elevated TLR/Nox4 expression. Conclusions: Our results suggested that puerarin inhibited oxidative stress and ferroptosis induced by I/R and, thus, delayed the progression of renal fibrosis, providing a new target for the treatment of renal fibrosis.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
29秒前
研友_LOoomL发布了新的文献求助10
35秒前
紫荆完成签到 ,获得积分10
40秒前
marceloclaro发布了新的文献求助30
58秒前
guoxihan完成签到,获得积分10
58秒前
kevinjiang完成签到,获得积分10
58秒前
善学以致用应助研友_LOoomL采纳,获得10
59秒前
Java完成签到,获得积分10
1分钟前
1分钟前
研友_LOoomL发布了新的文献求助10
1分钟前
在水一方应助淳于冬莲采纳,获得10
1分钟前
研友_LOoomL发布了新的文献求助10
2分钟前
管靖易完成签到 ,获得积分10
2分钟前
无辜的行云完成签到 ,获得积分0
2分钟前
共享精神应助研友_LOoomL采纳,获得10
2分钟前
2分钟前
稳重傲晴完成签到 ,获得积分10
2分钟前
淳于冬莲发布了新的文献求助10
2分钟前
DJ_Tokyo完成签到,获得积分10
2分钟前
3分钟前
3分钟前
研友_LOoomL发布了新的文献求助10
3分钟前
liuyingxin发布了新的文献求助10
3分钟前
liuliu完成签到 ,获得积分10
3分钟前
共享精神应助研友_LOoomL采纳,获得10
3分钟前
3分钟前
3分钟前
研友_LOoomL发布了新的文献求助10
3分钟前
白昼の月完成签到 ,获得积分0
4分钟前
liuyingxin完成签到,获得积分20
4分钟前
研友_LOoomL发布了新的文献求助10
4分钟前
顾矜应助liuyingxin采纳,获得10
4分钟前
深情安青应助研友_LOoomL采纳,获得10
4分钟前
5分钟前
不晓天发布了新的文献求助10
5分钟前
小马甲应助不晓天采纳,获得10
5分钟前
5分钟前
研友_LOoomL发布了新的文献求助10
5分钟前
5分钟前
搜集达人应助研友_LOoomL采纳,获得10
5分钟前
高分求助中
歯科矯正学 第7版(或第5版) 1004
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Semiconductor Process Reliability in Practice 720
GROUP-THEORY AND POLARIZATION ALGEBRA 500
Mesopotamian divination texts : conversing with the gods : sources from the first millennium BCE 500
Days of Transition. The Parsi Death Rituals(2011) 500
The Heath Anthology of American Literature: Early Nineteenth Century 1800 - 1865 Vol. B 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3229760
求助须知:如何正确求助?哪些是违规求助? 2877260
关于积分的说明 8198664
捐赠科研通 2544727
什么是DOI,文献DOI怎么找? 1374636
科研通“疑难数据库(出版商)”最低求助积分说明 647015
邀请新用户注册赠送积分活动 621836