Unveiling threats to glacier biota: Bioaccumulation, mobility, and interactions of radioisotopes with key biological components

Contemporary melting glaciers are considered a secondary source of pollutants including radionuclides. Cryoconite - biogenic sediment on the glacier surface - exhibits high concentrations of natural and anthrophogenic radioisotopes. Understanding the interactions between radioisotopes and organisms is essential for evaluating their potential impact on glacier-related ecosystems. Using a multidimensional approach and intensive sampling (17 glaciers), we investigated the relationships between activity concentrations of 137Cs and 210Pb and various biotic components of cryoconite such as the amount of organic matter, chlorophyll concentration, the ratio of cyanobacteria to all bacteria, and size of cryoconite granules. Additionally, to better understand the bioavailability and fate of radioisotopes in this ecosystem, we measured the uptake ratio of 137Cs, 210Pb, 238Pu, and 239+240Pu in the top consumers, and examined the mobility of radioisotopes by measuring 137Cs and 210Pb activity concentrations after a parallel extraction using media with different specific ion exchange capacities. The activity concentrations of both 137Cs and 210Pb showed a large variability reaching 5.8 kBq kg-1 and 7.2 kBq kg-1, respectively. Their activity concentrations were positively related to the amount of organic matter, however, the 210Pb also increased with the chlorophyll concentration. This might be due to the difference in the deposition of both elements: lead, being deposited constantly, binds also to currently developing communities, while caesium deposition peaked in the 1960s. The mobility analysis revealed that the loosely bound fraction of 210Pb was more strongly related to organic-metallic complexes than 137Cs. Firmly bound radioisotope fractions (anhydrous interlayer sites of minerals) were three times higher for 137Cs than for 210Pb. The median uptake ratios of radioisotopes were determined as 0.07-0.111 for 137Cs, 0.177 for 210Pb, 0.07 for 239+240Pu. Our findings emphasize the importance of organisms in the accumulation of radioisotopes on glaciers and suggest an impact of radionuclides on glacier organisms.