Development of air-assisted atomization device for the delivery of cells in viscous biological ink prepared with sodium alginate

生理盐水 移植 材料科学 活力测定 粘度 哈卡特 沉淀 生物医学工程 悬挂(拓扑) 沉积(地质) 海藻酸钠 复合材料 色谱法 化学工程 纳米技术 外科 细胞 化学 医学 细胞培养 环境工程 环境科学 生物 麻醉 冶金 数学 遗传学 古生物学 生物化学 同伦 沉积物 纯数学 工程类
作者
Xintao Yan,Ce Wang,Yuting Ma,Yao Wang,Feifei Song,Jinfeng Zhong,Xiangnan Wu
出处
期刊:Review of Scientific Instruments [American Institute of Physics]
卷期号:94 (4)
标识
DOI:10.1063/5.0102035
摘要

Skin wounds, especially large-area skin trauma, would bring great pain and even fatal risk to patients. In recent years, local autologous cell transplantation has shown great potential for wound healing and re-epithelialization. However, when the cell suspension prepared with normal saline is delivered to the wound, due to its low viscosity, it is easy to form big drops in the deposition and lose them from the wound bed, resulting in cell loss and uneven coverage. Here, we developed a novel air-assisted atomization device (AAAD). Under proper atomization parameters, 1% (w/v) sodium alginate (SA) solution carrier could be sprayed uniformly. Compared with normal saline, the run-off of the SA on the surface of porcine skin was greatly reduced. In theory, the spray height of AAAD could be set to achieve the adjustment of a large spray area of 1–12 cm2. In the measurement of droplet velocity and HaCaT cell viability, the spray height of AAAD would affect the droplet settling velocity and then the cell delivery survival rate (CSR). Compared with the spray height of 50 mm, the CSR of 100 mm was significantly higher and could reach 91.09% ± 1.82% (92.82% ± 2.15% in control). For bio-ink prepared with 1% (w/v) SA, the viability remained the same during the 72-h incubation. Overall, the novel AAAD uniformly atomized bio-ink with high viscosity and maintained the viability and proliferation rate during the delivery of living cells. Therefore, AAAD has great potential in cell transplantation therapy, especially for large-area or irregular skin wounds.
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