Dissociation Constant (Kd) Measurement for Small‐Molecule Binding Aptamers: Homogeneous Assay Methods and Critical Evaluations

Since 1990, numerous aptamers have been isolated and discovered for use in various analytical, biomedical, and environmental applications. This trend continues to date. A critical step in the characterization of aptamer binding is to measure its binding affinity toward both target and non-target molecules. Dissociation constant (K