Metastasis‐associated protein 1 participates in regulating luminal acidification of the epididymis via repressing estrogen receptor alpha transcription

Abstract Background As a component of the nucleosome remodeling and deacetylating (NuRD) complex, metastasis‐associated protein 1 (MTA1) has been reported to be abundant in male reproductive system and might participate in spermatogenesis and sperm maturation, whereas the precise functional role of MTA1 in these processes is still undetermined. Objective To investigate the effect and potential function of MTA1 in male fertility. Materials and methods Mta1 knockout mice ( Mta1 −/− ) were employed to detect their reproductive phenotype. The pH value of Mta1 −/− epididymal luminal fluid was measured, and the potential mechanism of MTA1 involved in regulating luminal acidification was detected in vivo and in vitro. A vasectomy model with abnormal pH of epididymal lumen was established to further detect the effect of MTA1 on epididymal luminal microenvironment. Results Mta1 −/− mice were fertile without any detectable defects in spermatogenesis or sperm motility while the deficiency of MTA1 could acidify the initial segment of epididymis to a certain extent. MTA1 could interact with estrogen receptor alpha (ERα) and inhibit the transcription of ERα target gene, hydrogen exchanger 3 (NHE3), and ultimately affect the epididymal luminal milieu. After vasectomy, the Mta1 −/− mice presented a more acidic epididymal lumen which was closer to the normal state compared to the wild‐type model. Discussion and conclusion MTA1 is dispensable for male fertility in mice, but plays a potentially important function in regulating luminal acidification of the epididymis.