大麻酚
酶
生物合成
生物化学
限制
氨基酸
化学
ATP合酶
鉴定(生物学)
生物
计算生物学
医学
机械工程
植物
大麻
精神科
工程类
作者
Yue Yang,Shimeng Liu,Zihe Li,Changlong Lai,Hao Wu,Zhenzhu Li,Wenhao Xia,Qiuhui Du,Lihui Huang,Wen Wang,Xiao Wang,Xianqing Chen
出处
期刊:Cannabis and cannabinoid research
[Mary Ann Liebert]
日期:2024-01-18
标识
DOI:10.1089/can.2023.0226
摘要
Introduction: Olivetolic acid (OLA) is a key intermediate in cannabidiol (CBD) synthesis, and cannabinoids are important neuroactive drugs. However, the catalytic activity of olivetolic acid synthase (OLS), the key enzyme involved in OLA biosynthesis, remains low and its catalytic mechanism is unclear. Materials and Methods: In this study, we conducted a scrupulous screening of the pivotal rate-limiting enzyme and analyzed its amino acid sites that are critical to enzyme activity as validated by experiments. Results: Through stringent enzyme screening, we pinpointed a highly active OLS sequence, OLS4. Then, we narrowed down three critical amino acid sites (I258, D198, E196) that significantly influence the OLS activity. Conclusions: Our findings laid the groundwork for the efficient biosynthesis of OLA, and thereby facilitate the biosynthesis of CBD.
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