微粒体
葡萄糖醛酸化
代谢物
羟基化
化学
体内
新陈代谢
药物代谢
代谢途径
色谱法
质谱法
生物化学
体外
生物
酶
生物技术
作者
Xinze Liu,Yiling Tang,Linhao Xu,Wei Liu,Ping Xiang,Taijun Hang,Hui Yan
摘要
Rationale ADB‐FUBIATA is one of the most recently identified new psychoactive substance (NPS) of synthetic cannabinoids. The co‐use of in vitro (human liver microsomes) and in vivo (zebrafish) models offers abundant metabolites and may give a deep insight into the metabolism of NPS. Methods In vivo and in vitro metabolic studies of new synthetic cannabinoid ADB‐FUBIATA were carried out using zebrafish and pooled human liver microsome models. Metabilites were structurally characterized by liquid chromatography–high‐resolution mass spectrometry. Results In total, 18 metabolites were discovered and identified in the pooled human liver microsomes and zebrafish, including seventeen phase I metabolites and one phase II metabolite. The main metabolic pathways of ADB‐FUBIATA were hydroxylation, dehydrogenation, N ‐dealkylation, amide hydrolysis, glucuronidation, and combination thereof. Conclusion Hydroxylated metabolites can be recommended as metabolic markers for ADB‐FUBIATA because of the structural characteristics and high intensity. These metabolism characteristics of ADB‐FUBIATA were useful for its further forensic or clinical related investigations.
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