Surface-enhanced infrared absorption spectroscopy (SEIRAS) for biochemical analysis: Progress and perspective

Advances in nanofabrication, instrumentation, miniaturization, and particularly the increased interdisciplinary dialogue between spectroscopists and their subscribers (biologists, environmentalists, and clinicians) have accelerated the application of surface-enhanced infrared absorption spectroscopy (SEIRAS) to biochemical analysis. This review outlines the basic principles of SEIRAS and its application to biochemical analysis in recent years. The review also comprehensively describes how SEIRAS can identify and quantify the biochemical activities of small molecules, biomacromolecules, and microorganisms, and summarizes the application trends of SEIRAS-based analytics and the value of SEIRAS information in modern biochemical applications and processes. Finally, it discusses the current challenges in SEIRAS-based biochemical analysis and proposes perspectives for further research in this field. Ultimately, the review aims to bridge the knowledge gap and promote further progress in SEIRAS-based biochemical analysis.