Optimizing Autotrophic Sulfide Oxidation in the Oxygen-Based Membrane Biofilm Reactor to Recover Elemental Sulfur

Biological sulfide oxidation is an efficient means to recover elemental sulfur (S0) as a valuable resource from sulfide-bearing wastewater. This work evaluated the autotrophic sulfide oxidation to S0 in the O2-based membrane biofilm reactor (O2-MBfR). High recovery of S0 (80–90% of influent S) and high sulfide oxidation (∼100%) were simultaneously achieved when the ratio of O2-delivery capacity to sulfide-to S0 surface loading (SL) (O2/S2– → S0 ratio) was around 1.5 (g O2/m2-day/g O2/m2-day). On average, most of the produced S0 was recovered in the MBfR effluent, although the biofilm could be a source or sink for S0. Shallow metagenomic analysis of the biofilm showed that the top sulfide-oxidizing genera present in all stages were Thauera, Thiomonas, Thauera_A, and Pseudomonas. Thiomonas or Pseudomonas was the most important genus in stages that produced almost only S0 (i.e., the O2/S2– → S0 ratio around 1.5 g of the O2/m2-day/g O2/m2-day). With a lower sulfide SL, the S0-producing genes were sqr and fccAB in Thiomonas. With a higher sulfide SL, the S0-producing genes were in the soxABDXYZ system in Pseudomonas. Thus, the biofilm community of the O2-MBfR adapted to different sulfide-to-S0 SLs and corresponding O2-delivery capacities. The results illustrate the potential for S0 recovery using the O2-MBfR.