材料科学
微流变学
韧性
间充质干细胞
粘弹性
干细胞
微尺度化学
纳米技术
复合材料
生物物理学
细胞生物学
数学
生物
数学教育
作者
Lihui Peng,Carlos Matellan,Minerva Bosch‐Fortea,Jordi Gonzalez‐Molina,Matteo Frigerio,Stefan Salentinig,Armando E. del Río Hernández,Julien E. Gautrot
标识
DOI:10.1002/adhm.202203297
摘要
Abstract Stem cells are known to sense and respond to the mechanical properties of biomaterials. In turn, cells exert forces on their environment that can lead to striking changes in shape, size and contraction of associated tissues, and may result in mechanical disruption and functional failure. However, no study has so far correlated stem cell phenotype and biomaterials toughness. Indeed, disentangling toughness‐mediated cell response from other mechanosensing processes has remained elusive as it is particularly challenging to uncouple Youngs' or shear moduli from toughness, within a range relevant to cell‐generated forces. In this report, it is shown how the design of the macromolecular architecture of polymer nanosheets regulates interfacial toughness, independently of interfacial shear storage modulus, and how this controls the expansion of mesenchymal stem cells at liquid interfaces. The viscoelasticity and toughness of poly( l ‐lysine) nanosheets assembled at liquid‐liquid interfaces is characterised via interfacial shear rheology. The local (microscale) mechanics of nanosheets are characterised via magnetic tweezer‐assisted interfacial microrheology and the thickness of these assemblies is determined from in situ ellipsometry. Finally, the response of mesenchymal stem cells to adhesion and culture at corresponding interfaces is investigated via immunostaining and confocal microscopy.
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